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Merck
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安全性情報

MABS1180

Sigma-Aldrich

Anti-E3 ubiquitin-protein ligase UBR1 Antibody, clone 6H9.1

clone 6H9.1, from mouse

別名:

EC: 2.3.2.27, N-recognin-1, RING-type E3 ubiquitin transferase UBR1, Ubiquitin-protein ligase E3-alpha-1, Ubiquitin-protein ligase E3-alpha-I

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

mouse

品質水準

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

6H9.1, monoclonal

交差性

human, rat

包装

antibody small pack of 25 μg

テクニック

immunohistochemistry: suitable (paraffin)
western blot: suitable

アイソタイプ

IgG2aκ

NCBIアクセッション番号

UniProtアクセッション番号

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... UBR1(197131)
rat ... Ubr1(499877)

詳細

E3 ubiquitin-protein ligase UBR1 (UniProt: Q8IWV7; also known as EC: 2.3.2.27, N-Recognin-1, RING-type E3 ubiquitin transferase UBR1, Ubiquitin-protein ligase E3-alpha-1, Ubiquitin-protein ligase E3-alpha-I) is encoded by the UBR1 gene (Gene ID: 197131) in human. E3 ubiquitin-protein ligase UBR1 is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It may also be involved in pancreatic homeostasis. Two isoforms of UBR1 have been described that are produced by alternative splicing. UBR1 is broadly expressed protein with highest levels reported in skeletal muscle, kidneys, and pancreas. UBR1 has two zinc finger domains. The UBR-type domain is localized to amino acids 97 to 168 and it forms a pocket that mediates recognition of type 1 N-degrons. It exhibits preference for arginine in first position and displays poor affinity for histidine. The second zinc finger domain, RING-type, atypical, is localized to amino acids 1098-1201. Mutations in UBR1 gene are causative factor in Johanson-Blizzard syndrome that is characterized by congenital exocrine pancreatic insufficiency, malformations such as nasal wing aplasia, and mental retardation.

特異性

Clone 6H9.1 detects E3 ubiquitin-protein ligase UBR1 in human and rat cells. It targets an epitope within 101 amino acids from the internal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 101 amino acids from the internal region of human E3 ubiquitin-protein ligase UBR1.

アプリケーション

Research Category
細胞シグナル伝達
Anti-E3 ubiquitin-protein ligase UBR1, clone 6H9.1, Cat. No. MABS1180, is a mouse monoclonal that detects E3 ubiquitin-protein ligase UBR1 and has been tested for use in Immunohistochemistry (Paraffin) Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected E3 ubiquitin-protein ligase UBR1 in human pancreas and human kidney tissue sections.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected E3 ubiquitin-protein ligase UBR1 in C6 cell lysate.

品質

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected E3 ubiquitin-protein ligase UBR1 in HEK293 cell lysate.

ターゲットの説明

~200 kDa observed; 200.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MABS1180-25UG:
MABS1180-100UG:


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