MABS1163

Anti-CSF-1R/CD115 Antibody clone FER216

clone FER216, from mouse

• 別名: Macrophage colony-stimulating factor 1 receptor, CD115, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: FER216, monoclonal
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_005202
• UniProtアクセッション番号: P07333
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CSF1R(1436)

詳細

詳細

Macrophage colony-stimulating factor 1 receptor (EC 2.7.10.1; UniProt P07333; also known as CD115, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms) is encoded by the CSF1R (also known as CSFR, FMS, HDLS) gene (Gene ID 1436) in human. CSF1R is a CSF1/PDGF family receptor tyrosine kinase that mediates macrophage colony stimulating factor 1 (M-CSF1) and IL-34 signaling by acting as the receptor for these two cytokines. CSF1R plays a key role in the differentiation, recruitment and activation of tissue macrophages. Tumor-associated macrophages (TAMs) have been associated with survival in classic Hodgkin lymphoma (cHL) and other lymphoma types. Hyperphosphorylated CSF1R is found in cHL tissue and highlights the importance of CSF1/CSF1R signaling in the recruitment of TAMs. CSF1R is initially produced with a signal peptide sequence (a.a. 1-19), the removal of which yields the mature type I single transmembrane protein with an extracellular region (a.a. 20-517), a transmembrane segment (a.a. a.a. 518-538), and a cytoplasmic region (a.a. 539-972) that contains the kinase domain (a.a. 582-910).

特異性

Clone FER216 reacts with the extracellular domain of human CSFR1, but not the extracellular domain of human FGFR1. Clone FER216 detected the ~100 kDa human CSFR1 in nomral tonsil and Hodgkin lymphoma (cHL) tissue extracts, as well as an additional ~150 kDa phosphorylated CSFR1 band in cHL tissue extracts (Martín-Moreno, A.M., et al. (2015). PLoS One. 2015 Jun 12;10(6):e0125203).

免疫原

Epitope: Extracellular domain.

Human IgG1 Fc-tagged recombinant human CSF-1R/CD115 extracellular domain.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
サイトカイン及びサイトカイン受容体

Anti-CSF-1R/CD115 Antibody clone FER216 is an antibody against CSF-1R for use in Western Blotting.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected CSF-1R/CD115 in 10 µg of human tonsil tissue lysate.

品質

Evaluated by Western Blotting in human colon tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CSF-1R/CD115 in 10 µg of human colon tissue lysate.

ターゲットの説明

~107 kDa observed. Uncharacterized band(s) may appear in some lysates.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable