MABN841

Anti-Semaphorin 4A Antibody, clone 8B5.1

clone 8B5.1, from mouse

• 別名: Semaphorin 4A, Semaphorin-B, Sema B, Sema4A
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 8B5.1, monoclonal
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgMκ
• NCBIアクセッション番号: NP_001180229
• UniProtアクセッション番号: Q9H3S1
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SEMA4A(64218)

詳細

詳細

Semaphorin-4A (UniProt Q9H3S1; also known as Sema B, Semaphorin-B) is encoded by the SEMA4A (also known as CORD10, RP35, SEMAB, SEMB) gene (ORP UNQ783/PRO1317; Gene ID 64218) in human. Semaphorin-4A is a membrane-type class IV semaphorin originally characterized for its role in axon guidance during neural development, Semaphorin-4A also plays a role in Th cells activation and the differentiation of Th1 and Th17 cells. In addition Semaphorin-4A is involved in angiogenesis and endothelial cells migration by modulating VEGF signaling. Consistent with its important role in Th cells, Semaphorin-4A level is reported to be elevated in the serum samples from patients with multiple sclerosis (MS) respond poorly to interferon-beta (IFN-β) therapy. Semaphorin-4A neutralizing antibodies are reported to inhibit antigen-specific T cell generation and ameliorate experimental autoimmune encephalomyelitis (EAE), an animal model of MS.

特異性

Expected to react with both spliced isoforms.

免疫原

Epitope: Sema domain

GST-tagged recombinant protein corresponding to the Sema domain of human Semaphorin 4A.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
発生シグナル伝達

This Anti-Semaphorin 4A Antibody, clone 8B5.1 is validated for use in Western Blotting for the detection of Semaphorin 4A.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Semaphorin 4A in 10 µg of human retinal pigment epithelia (RPE) ARPE-19.

品質

Evaluated by Western Blotting in human monocytic leukemia THP-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Semaphorin 4A in 10 µg of human monocytic leukemia THP-1 cell lysate.

ターゲットの説明

~85 kDa observed. 83.6 kDa (isoform 1) and 69.1 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

物理的形状

Format: Purified

Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable