MABN318

Anti-Transmembrane Prostatic Acid Phosphatase Antibody/TMPAP, clone 3G10.1

clone 3G10.1, from mouse

• 別名: Prostatic acid phosphatase, PAP, 5′-nucleotidase, 5′-NT, Ecto-5′-nucleotidase, Thiamine monophosphatase, Transmembrane Prostatic Acid Phosphatase, TMPase, ACPP, TMPAP
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 3G10.1, monoclonal
• 交差性: human
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_001127666
• UniProtアクセッション番号: P15309
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ACPP(55)

詳細

詳細

ACPP, also known as Prostatic acid phosphatase, or PAP, 5′-nucleotidase (5′-NT), Ecto-5′-nuclotidase, or Thiamine monophosphatase (TMPase) , and encoded by the gene ACPP, is a non-specific tyrosine phosphatase that has both a secreted form and a cell membrane bound form. ACPP dephosphorylates a diverse number of substrates including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. ACPP also has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Additionally, the membrane bound form of ACPP has ecto-5′-nucleotidase activity in dorsal root ganglion (DRG) neurons and acts as a pain suppressor. Finally ACPP can also act as a tumor suppressor of prostate cancer. ACPP isoform 1is secreted and isoform 2 is membrane bound. Isoform 2 appears to shuttle between the cell membrane, lysosomes, and intracellular vesicles. ACPP is expressed in many tissues and is used as a marker in various stages of prostatic cancer diagnostics. Also the enzyme was intensely studied in HIV infection because it can be proteolytically cleaved in seminal fluid to produce several peptides (SEVI peptides) one of which, PAPf39, happens to enhance HIV infection by binding HIV virions and helping to attach them to target cells--interestingly such SEVI peptides are degraded in vitro by EGCG, a compound found in green tea extract.

特異性

This antibody is expected to cross react with isoform 2 based upon immunogen design.

免疫原

GST-tagged recombinant protein corresponding to human Transmembrane Prostatic Acid Phosphatase/TMPAP.

アプリケーション

Research Category
ニューロサイエンス

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Transmembrane Prostatic Acid Phosphatase/TMPAP in human prostate tissue.

This Anti-Transmembrane Prostatic Acid Phosphatase Antibody/TMPAP, clone 3G10.1 is validated for use in western blotting & IHC (Paraffin) for the detection of Transmembrane Prostatic Acid Phosphatase/TMPAP.

品質

Evaluated by Western Blotting in human prostate tumor tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected this antibody detected Transmembrane Prostatic Acid Phosphatase/TMPAP in 200 µg of human prostate tumor tisssue lysate.

ターゲットの説明

~48 kDa observed. Two isoforms are known to exist at 45 kDa (isoform 1) and 48 kDa (isoform 2). Uncharacterized band(s) may be observed in some cell lysates.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable