MABN2527

Anti-Aquaporin 4 Antibody

• 別名: AQP4 Antibody, Anti-AQP4
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: E5415A, monoclonal
• 分子量: calculated mol wt 34.44 kDa; observed mol wt ~45 kDa
• 化学種の反応性: mouse, rat
• 包装: antibody small pack of 100 μg
• テクニック: ELISA: suitable; activity assay: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• UniProtアクセッション番号: P55088
• 輸送温度: dry ice
• 保管温度: -10 to -25°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Aqp4(11829)

詳細

詳細

Aquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AGP4 gene (Gene ID: 361) in human. Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis Optica (NM) from multiple sclerosis. Clone mCCD does not cause redistribution of Aquaporin-4 and also it does not affect coaggregation astrocytic CD32-B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl. Acad. Sci. USA 114(21); 5491-5496; Huang, P., et. al. (2016). Biochem Biophys Rep. 7:77-83).

特異性

Clone E5415A is a mouse monoclonal antibody generated in AQP-4 knockout mice that detects murine Aquaporin-4. It targets an epitope within the extracellular domain. It detects both M1 (Isoform 2) and M23 (Isoform 1).

免疫原

Recombinant extracellular domain of murine Aquaporin 4.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in Mouse heart tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Aquaporin 4 in Mouse heart tissue lysate.

Tested Applications

Function Analysis: A representative lot caused a loss of AQP4 in an experimental autoimmune encephalomyelitis (EAE) rat model in a dose dependent manner and enhanced endocytosis of endogenous AQP4 in astrocytes. (Kurosawa, K., et. al. (2015). Acta Neuropathol Commun. 3:82; H uang, P., et. al. (2016). Biochem Biophys Rep. 7:77-83) .

ELISA Analysis: A representative lot detected Aquaporin 4 in ELISA applications (Huang, P., et. al. (2016). Biochem Biophys Rep. 7:77-83).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Aquaporin 4 in mouse astrocytes.

Western Blotting Analysis: A representative lot detected Aquaporin 4 in Western Blotting applications (Huang, P., et. al. (2016). Biochem Biophys Rep. 7:77-83).

Immunofluorescence Analysis: A representative lot detected Aquaporin 4 in Immunofluorescence applications (Huang, P., et. al. (2016). Biochem Biophys Rep. 7:77-83).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Aquaporin 4, clone E5415A, Cat. No. MABN2527, is a mouse monoclonal antibody that detects Aquapoorin-4 and is tested for use in ELISA, Function Analysis, Immunocytochemistry, Immunofluorescence, and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG2a in PBS without azide.

保管および安定性

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable