MABN1797

Anti-Prdx-6 Antibody, clone TAG-2A12

clone TAG-2A12, from mouse

• 別名: Peroxiredoxin-6, EC: 1.11.1.15, 1-Cys peroxiredoxin, 1-Cys PRX, 24 kDa protein, Acidic calcium-independent phospholipase A2, aiPLA2, Antioxidant protein 2, Liver 2D page spot 40, Non-selenium glutathione peroxidase, NSGPx
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: TAG-2A12, monoclonal
• 化学種の反応性: human
• 包装: antibody small pack of 25 μg
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_004896
• UniProtアクセッション番号: P30041
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PRDX6(9588)

詳細

詳細

Peroxiredoxin-6 (UniProt: P30041; also known as EC: 1.11.1.15, 1-Cys peroxiredoxin, 1-Cys PRX, 24 kDa protein, Acidic calcium-independent phospholipase A2, aiPLA2, Antioxidant protein 2, Liver 2D page spot 40, Non-selenium glutathione peroxidase, NSGPx) is encoded by the PRDX6 (also known as AOP2, KIAA0106) gene (Gene ID: 9588) in human. Peroxiredoxin-6 is a cytoplasmic protein that is involved in redox regulation of the cell. It reduces hydrogen peroxide and short chain organic fatty acid, and phospholipid hydroperoxides. It is the only mammalian 1-Cys member of the Prdx family with bifunctional - glutathione peroxidase and phospholipase A2 activities. Peroxiredoxin-6 shares several structural and functional properties with other members of the Prdx family, however, it has only one conserved cysteine residue and its thioredoxin does not participate in the catalytic cycle. Peroxiredoxin-6 is expressed in higher levels in epithelium such as apical regions of respiratory epithelium and skin epidermis and within the lung, it is expressed at relatively high levels within the type II alveolar epithelial cells and bronchiolar Clara cells. Lower levels of expression are reported in alveolar macrophages and microvascular endothelium. Human peroxiredoxin-6 shares 87% sequence identity with mouse, rat, and bovine.

特異性

Clone TAG-2A12 detects Peroxiredoxin-6 in human cells.

免疫原

Corneal endothelial cells (hCENC) isolated from corneas of human cadavers and immunized with monophosphoryl-lipid A trehalose dicorynomycolate (MPLCTDM) adjuvant.

アプリケーション

Research Category
ニューロサイエンス

Anti-Prdx-6 Antibody, clone TAG-2A12, Cat. No. MABN1797, is a highly specific mouse monoclonal antibody that targets Prdx-6 and has been tested in Flow Cytometry, Immunofluorescence, Immunocytochemistry and Immunoprecipitation, and Western Blotting.

Immunoprecipitation Analysis: A representative lot detected Prdx-6 in Immunoprecipitation applications (Ding, V., et al. (2014). MAbs. 6(6):1439-52).

Immunocytochemistry Analysis: A representative lot detected Prdx-6 in Immunocytochemistry applications (Ding, V., et al. (2014). MAbs. 6(6):1439-52).

Flow Cytometry Analysis: A representative lot detected Prdx-6 in Flow Cytometry applications (Ding, V., et al. (2014). MAbs. 6(6):1439-52).

Immunofluorescence Analysis: A representative lot detected Prdx-6 in Immunofluorescence applications (Ding, V., et al. (2014). MAbs. 6(6):1439-52).

Western Blotting Analysis: A representative lot detected Prdx-6 in Western Blotting applications (Ding, V., et al. (2014). MAbs. 6(6):1439-52).

Flow Cytometry Analysis: A representative lot detected Prdx-6 in human corneal endothelial cells from a donor. (Courtesy of Dr. Vanessa Ding, Ph.D. and Dr. Andre Choo, Ph.D, BTI, Singapore).

品質

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be human IgG1 .

ターゲットの説明

~28 kDa observed; 25.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1