MABN110

Anti-pro-BDNF Antibody, clone 5D4.1

clone 5D4.1, 1 mg/mL, from mouse

• 別名: Brain-derived neurotrophic factor, Abrineurin
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 5D4.1, monoclonal
• 交差性: mouse, human, rat
• 濃度: 1 mg/mL
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_001137277
• UniProtアクセッション番号: P23560
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... BDNF(627)

詳細

詳細

Brain Derived Neurotrophic Factor (BDNF) is a secreted protein that promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer’s and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF exists as monomers or homodimers and binds to TRKB/NTRK2 receptors.

特異性

The antibody reacts to the pro form of BDNF

免疫原

GST-tagged recombinant protein corresponding to Human pro-BDNF.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
ニューロン発生

This Anti-pro-BDNF Antibody, clone 5D4.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of pro-BDNF.

Western Blotting Analysis: A 0.5 µg/mL dilution from a representative lot detected 10 µg of recombinant protein Pro-BDNF but not recombinant protein BDNF.
Immunohistochemistry Analysis: A 1:2000 dilution from a representative lot detected Pro-BDNF in human cerebral cortex and thalamic tissue.

品質

Evaluated by Western Blotting in Rat Brain cell lysate.

Western Blotting Analysis: A 0.5 µg/mL diultion of this antibody detected pro-BDNF in 200 µg of Rat Brain cell lysate.

ターゲットの説明

~ 34 kDa observed (Neurochemical Journal, 2012, Vol. 6, No. 4, pp 260-264).

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable