MABF917

Anti-CRACC Antibody, clone 4G2

clone 4G2, from rat

• 別名: SLAM family member 7, CD2-like receptor-activating cytotoxic cells, CRACC, Leukocyte cell-surface antigen, Novel Ly9, CD319
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 4G2, monoclonal
• 化学種の反応性: mouse
• テクニック: flow cytometry: suitable; immunoprecipitation (IP): suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_653122.2
• UniProtアクセッション番号: Q8BHK6
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Slamf7 (75345)

詳細

詳細

SLAM family member 7 (UniProt Q8BHK6; also known as CD2-like receptor-activating cytotoxic cells, CRACC, Leukocyte cell-surface antigen, Novel Ly9, CD319) is encoded by the Slamf7 (also known as Cs1) gene (Gene ID 75345) in murine species. CRACC/CS1 belongs to the 6-member (SLAM/CD150, Ly-9/CD229, 2B4/CD244, CD84, NTB-A/CD352, CRACC) signaling lymphocytic activation molecule family (SLAMF) of receptors broadly expressed in hematopoietic cells. 2B4 and CD48 interact with each other, while rest of the family members act as self-ligands, where two identical molecules on the surface of two different cells can interact as receptor and ligand to each other. CRACC is synthesized with a signal peptide sequence (a.a. 1-22), the removal of which yields the mature CRACC with an extracellular region (a.a. 23-224) that contains a V-type and a C2-type Ig-like domain (a.a. 27-112 and 128-203), followed by a single transmembrane domain (a.a. 225-245) and a cytoplasmic tail (a.a. 246-333). CRACC cytoplasmic domain contains one immunoreceptor tyrosine-based switch motif (ITSM; 278-TFYSTV-283, numbering based on mature murine sequence), which upon phosphorylation (pY300) recruits SAP family adaptor EWS-Fli1-activated transcript-2 (EAT-2) for signaling transduction. CRACC can also be phosphorylated on Tyr280. Unlike pY300, pY280 mediates the inhibitory function of CRACC in NK cells. In addition to NK cells, evidence indicates that CRACC is also involved in regulating B- and T-cell functions.

特異性

Clone 4G2 reacted with murine T lymphoma BI-141 transfectants expressing mouse CRACC but not with those transfected with other members of the SLAM family (CD84, 2B4, Ly9, Ly108 or SLAM) (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305). Expected to react with all four spliced isoforms of murine CRACC reported by UniProt (Q8BHK6).

免疫原

Epitope: Extracellular domain.

Human IgG1 Fc -tagged recombinant mouse CRACC extracellular domain.

アプリケーション

Research Category
炎症及び免疫

Research Sub Category
免疫シグナル伝達

Anti-CRACC Antibody, clone 4G2 is an antibody against CRACC for use in Flow Cytometry, Affects Function, Immunoprecipitation.

Flow Cytometry Analysis: A representative lot immunostained murine T lymphoma BI-141 transfectants expressing mouse CRACC but not those transfected with other members of the SLAM family (CD84, 2B4, Ly9, Ly108 or SLAM) (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
Flow Cytometry Analysis: A representative lot detected CRACC expression on the surface of many types of mouse immune system cells, including NK cells, NKT cells, B cells, DCs, macrophages and activated T cells (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
Affects Function: A representative lot, in combination with a rabbit anti-rat IgG, cross-linked cell surface CRACC and induced similar CRACC phosphorylation in IL-2-activated NK cells from wild-type, SAP-deficient, or EAT-2–ERT–double knock (DKO) mice (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
Immunoprecipitation Analysis: A representative lot, in combination with a rabbit anti-rat IgG and protein A, immunoprecipitated CRACC from lysates of IL-2-activated mouse NK cells (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).

品質

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 0.1 µg of this antibody detected CRACC-positive mouse splenocytes.

ターゲットの説明

34.77 kDa (isoform 1), 34.87 kDa (isoform 2), 30.71 kDa (isoform 3), and 30.61 kDa (isoform 4) calculated.

物理的形状

Protein G Purified

Format: Purified

Purified rat monoclonal IgG1κ antibody in PBS without preservatives.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable