MABF444

Anti-CD45R (B220) Antibody (human/mouse), PE-Cy5, clone RA3-6B2

clone RA3-6B2, 0.2 mg/mL, from rat

• 別名: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 100
• 結合体: PE-Cy5
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: RA3-6B2, monoclonal
• 化学種の反応性: mouse, human
• 濃度: 0.2 mg/mL
• テクニック: flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• UniProtアクセッション番号: P06800
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PTPRC(5788) ; mouse ... Ptprc(19264)

詳細

詳細

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.

免疫原

Immunogen information for this antibody is proprietary information.

アプリケーション

Research Category
炎症及び免疫

This rat monoclonal antibody Anti-CD45R (B220) (human/mouse), PE-Cy5, clone RA3-6B2 validated for use in western blotting, IP, IHC, Immunofluorescence & Flow Cytometry for the detection of CD45R (B220).

品質

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 µg of this antibody detected CD45R (B220) in one million C57Bl/6 splenocytes.

物理的形状

Size Exclusion Chromatography

Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

保管および安定性

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable