MABF2791

Anti-CCR9 Antibody, clone 7E7

• 別名: C-C chemokine receptor type 9;C-C CKR-9;CC-CKR-9;CCR-9;Chemokine C-C receptor 10;CDw199
• UNSPSCコード: 12352203
• NACRES: NA.43
• クローン: 7E7, monoclonal
• application: FACS; IF; IHC (p)
• 化学種の反応性: mouse, human
• テクニック: flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

特性

• 由来生物: rat
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 7E7, monoclonal
• 分子量: calculated mol wt 41.91 kDa
• 精製方法: using protein G
• 化学種の反応性: mouse, human
• 包装: antibody small pack of 100 μg
• テクニック: flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• アイソタイプ: IgG2b
• エピトープ配列: N-terminal
• タンパク質IDアクセッション番号: NP_001160097
• UniProtアクセッション番号: Q9WUT7
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TACSTD2(4070)

詳細

詳細

C-C chemokine receptor type 9 (UniProt: Q9WUT7; also known as C-C CKR-9, CC-CKR-9, CCR-9, Chemokine C-C receptor 10, CDw199) is encoded by the Crr9 (also known as Cmkbr10) gene (Gene ID: 12769) in murine species. CCR9 is a multi-pass membrane protein of the G-protein coupled receptor 1 family. It has four extracellular domains, seven transmembrane domains, and four cytoplasmic domains and serves as a receptor for chemokine CCL25/TECK. It is highly expressed in the thymus tissue. It is also expressed on virtually all small intestinal T cells and IgA-producing plasma cells (PCs) from the spleen. It is reported to be crucial for the positioning of PCs to the small intestine. Higher expression of CCR9 is observed in newly generated PCs and it is down-regulated once these cells reach their final destination in the intestine. In wild-type mice, a majority of the PCs are shown to locate to the lower half of the villus where CCL25 is also expressed at high levels. CCR9 deficient mice exhibit reduced number of PCs in the intestine and are unable to mount a T cell dependent antigen specific IgA response. CCR9-/- hematopoietic stem cells are deficient in their ability to generate all major thymocyte subsets. (Ref.: Svensson, M., et al. (2008). J. Leukoc. Biol. 83(1); 156-164; Pabst, O., et al. (2004). J. Exp. Med. 199(3); 411-416).

特異性

Clone 7E7 is a rat monoclonal antibody that detects C-C chemokine receptor type 9 (CCR9). It targets an epitope within 20 amino acids from the fist extracellular domain in the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the first extracellular domain in the N-terminal region of mouse C-C-chemokine receptor type 9 (CCR9).

アプリケーション

Quality Control Testing

Evaluated by Flow Cytometry in BALB/c mouse thymocytes.

Flow Cytometry Analysis: 1 μg of this antibody detected CCR9 in one million BALB/c mouse thymocytes.

Tested Applications

Immunohistochemistry Applications: A representative lot detected CCR9 in Immunohistochemistry applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15).

Flow Cytometry Analysis: A representative lot detected CCR9 in Flow Cytometry applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15; Svensson, M., et al. (2008). J Leukoc Biol. 83(1):156-64; Chennupati, V., et al. (2010). J Immunol. 185(9):5160-8).

Immunofluorescence Analysis: A representative lot detected CCR9 in Immunofluorescence applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CCR9, clone 7E7, Cat. No. MABF2791, is a rat monoclonal antibody that detects CCR9 and is tested for use in Flow Cytometry, Immunofluorescence, and Immunohistochemistry.

物理的形状

Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1