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由来生物
rat
品質水準
結合体
unconjugated
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
20D5, monoclonal
分子量
observed mol wt ~27.68 kDa
化学種の反応性
mouse
包装
antibody small pack of 100 μL
テクニック
flow cytometry: suitable
inhibition assay: suitable
アイソタイプ
IgG2a
UniProtアクセッション番号
輸送温度
dry ice
保管温度
-10 to -25°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... Klrc1(16641)
詳細
Killer cell lectin-like receptor subfamily C, member 1/2/3 (UniProt: Q9Z202/ Q9WTJ9/Q9QXN7; also known as Natural killer cell protein group 2-A1/ Natural killer cell protein group 2-C1, Natural killer cell receptor NKG2A/Natural killer cell receptor NKG2C/ Natural killer cell group 2E cell receptor NKG2E) are encoded by the Klrc1/2/3 genes (Gene IDs: 16641/ 16642/58179) in murine species. The NKG2 proteins are members of the lectin-like receptor family that heterodimerize with CD94 on the surface of NK and NKT cells. They are also shown to be expressed on activated CD8+ T cells. They are integral component of membrane and are present on the external side of plasma membrane. Within their extracellular carbohydrate recognition domains, NKG2A, NKG2C and NKG2E share extensive homology; however, NKG2C and NKG2E receptors differ significantly from NKG2A in their cytoplasmic domains. NKG2/CD94 heterodimeric complexes are shown to recognize Qa-1 that is described as a nonclassical MHC class I antigen. These heterodimers are reported to transduce signals following ligand binding. NKG2A is shown to be inhibitory in nature, while NKG2C and NKG2E transduce stimulatory signals. Comparatively, NKG2A is more abundant than NKG2C and NKG2E in NK cells. Murine NKG2A contains two intracytoplasmic sequences that closely resemble the Immunoreceptor Tyrosine- based Inhibitory Motif (ITIM). Clone 20D5 can be used for the identification of NK cells expressing functional CD94/NKG2 receptors and is suitable for blocking NKG2A signals in murine tumor models. (Ref.: Vance, RE., et al. (1999). J. Exp. Med. 190(12); 1801-1812).
特異性
Clone 20D5 is a rat monoclonal antibody that detects murine Killer cell lectin-like receptor subfamily C (NKG2 A, C and E). It targets an epitope within the extracellular domain.
免疫原
Stable Chinese hamster ovary (CHO) cells expressing the B6 alleles of CD94 and NKG2A.
アプリケーション
Quality Control Testing
Evaluated by Flow Cytometry in C57BL/6 mouse splenocytes.
Flow Cytometry Analysis: 1 μg of this antibody detected NKG2A/C/E in one million C57BL/6 mouse splenocytes.
Tested Applications
Flow Cytometry Analysis: A representative lot detected NKG2A/C/E in Flow Cytometry applications (Vance, R.E., et. al. (1999). J Exp Med. 190(12):1801-12).
Inhibition Analysis: A representative lot of this antibody completely blocked all binding of Qa-1 tetramers to NK1.11 splenocytes. (Vance, R.E., et. al. (1999). J Exp Med. 190(12):1801-12).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Flow Cytometry in C57BL/6 mouse splenocytes.
Flow Cytometry Analysis: 1 μg of this antibody detected NKG2A/C/E in one million C57BL/6 mouse splenocytes.
Tested Applications
Flow Cytometry Analysis: A representative lot detected NKG2A/C/E in Flow Cytometry applications (Vance, R.E., et. al. (1999). J Exp Med. 190(12):1801-12).
Inhibition Analysis: A representative lot of this antibody completely blocked all binding of Qa-1 tetramers to NK1.11 splenocytes. (Vance, R.E., et. al. (1999). J Exp Med. 190(12):1801-12).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-NKG2A/C/E, clone 20D5, Cat. No. MABF2689, is a rat monoclonal antibody that detects Killer cell lectin-like receptor subfamily C, member 1/2/3 and is tested for use in Flow Cytometry and Inhibition Assay.
物理的形状
Purified rat monoclonal antibody IgG2a in PBS without azide.
保管および安定性
Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABF2689-100UL:
MABF2689-25UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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