MABF2302

Anti-HLA-A02:01 EBV-LMP2A Antibody, clone MAB243

• 別名: Latent membrane protein 2
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: MAB243 (also referenced as L2), monoclonal
• 分子量: calculated mol wt 53.01 kDa
• 精製方法: using protein G
• 化学種の反応性: human
• 包装: antibody small pack of 100 μg
• テクニック: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: C-terminal
• タンパク質IDアクセッション番号: Unknown
• UniProtアクセッション番号: P13285
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... LMP2(3783751)

詳細

詳細

Latent membrane protein 2 (UniProt: P13285; also known as Terminal protein) is encoded by the LMP2 gene (Gene ID: 3783751) in Epstein-Barr virus. EBV is a gamma-herpesvirus, a causative agent of infectious mononucleosis. In healthy individuals, EBV typically establishes a persistent latent infection in which the virus can be detected in resting, nonproliferating peripheral B-lymphocytes. These latently infected cells express only 2 virally encoded genes, LMP2A and EBNA1. Although the majority of virus infected individuals remain healthy, EBV has been implicated in the pathogenesis of African Burkitt s lymphoma and nasopharyngeal carcinoma. LMP2A, a multi-pass membrane protein, localizes in plasma membrane lipid rafts. It maintains EBV latent infection of B cells by preventing lytic reactivation of the virus in response to surface immunoglobulin (sIg) cross-linking. It acts like a dominant negative inhibitor of the sIg-associated protein tyrosine kinases, LYN and SYK. It is also reported to block the translocation of the B-cell antigen receptor (BCR) into lipid rafts, which prevents the subsequent signaling and accelerated internalization of the BCR upon BCR cross-linking. It also serves as a molecular scaffold to recruit SYK, LYN and E3 protein-ubiquitin ligases leading to ubiquitination and degradation of these tyrosine kinases. LMP2A also exhibits a constitutive signaling activity in non-transformed cells, inducing bypass of normal B lymphocyte developmental checkpoints, which allows immunoglobulin-negative cells to colonize peripheral lymphoid organs. (Ref.: Lai, J., et al. (2017). Sci. Rep. 7; 9923; Rancan, C., et al. (2015). PLoS Pathol. 11(6); e1004906).

特異性

Clone MAB423 (L2) is a mouse monoclonal antibody that detects EBV-LMP2A. It targets an epitope within 9 amino acids from the C-terminal region.

免疫原

HLA-A201 with EBV (strain B95-8) LMP2A (CLGGLLTMV) 426-434 recombinant protein.

アプリケーション

Quality Control Testing

Evaluated by Flow Cytometry in T2 cells pulsed with LMP2 peptide (426-434).

Flow Cytometry Analysis (FC): 1 μg of this antibody detected HLA-A2-EBV LMP2A in one million T2 cells pulsed with LMP2 peptide (426-434).

Tested Applications

ELISA Analysis: A representative lot detected HLA-A02:01 EBV-LMP2A in ELISA applications (Lai, J., et al. (2016). Blood. 128(10):1396-407; Lai, J., et al. (2017). Sci Rep. 7(1):9923).

Immunohistochemistry Applications: A representative lot detected HLA-A02:01 EBV-LMP2A in Immunohistochemistry applications (Lai, J., et al. (2016). Blood. 128(10):1396-407).

Western Blotting Analysis: A representative lot detected HLA-A02:01 EBV-LMP2A in Western Blotting applications (Lai, J., et al. (2016). Blood. 128(10):1396-407).

Flow Cytometry Analysis: A representative lot detected HLA-A02:01 EBV-LMP2A in Flow Cytometry applications (Lai, J., et al. (2016). Blood.;128(10):1396-407; Lai, J., et al. (2017). Sci Rep.;7(1):9923).

Immunofluorescence Analysis: A representative lot detected HLA-A02:01 EBV-LMP2A in Immunofluorescence applications (Lai, J., et al. (2016). Blood. 128(10):1396-407).

Immunocytochemistry Analysis: A representative lot detected HLA-A02:01 EBV-LMP2A in Immunocytochemistry applications (Lai, J., et al. (2016). Blood. 128(10):1396-407).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-HLA-A02:01 EBV-LMP2A, clone MAB243, Cat. No. MABF2302, is a mouse monoclonal antibody that detects EBV-LMP2A and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable