MABF2287

Anti-IL-13 Antibody, clone 6D11.8.11

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 6D11.8.11, monoclonal
• 分子量: calculated mol wt 15.82 kDa; observed mol wt ~12 kDa
• 精製方法: using protein G
• 化学種の反応性: human
• 包装: antibody small pack of 100 μg
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: Unknown
• タンパク質IDアクセッション番号: NP_002179.2
• UniProtアクセッション番号: P35225
• 輸送温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Interleukin-13 (UniProt: P35225; also known as IL-13) is encoded by the IL13 (also known as NC30) gene (Gene ID: 3596) in human. IL-13 is an extracellular/secreted glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form. IL-13 is an immunoregulatory cytokine that is released predominantly by CD4+ and CD8+ T cells and by mast cells and eosinophils and regulates the function of human B cells and monocytes. It is closely related to IL-4 in its biological function but, in general, the responses of cells to IL-13 appear to be smaller in magnitude. Both IL-4 and IL-13, independently of each other, are able to induce IgE antibody production. The intracellular site of storage of IL-13 in eosinophils is associated with the crystalloid granules, indicating that IL-13 is a preformed mediator and is released upon stimulation of degranulation. IL-13 signaling is mediated through a shared multi-subunit heterodimeric receptor complex consisting of IL-4R and IL-13R1. This heterodimerization activates STAT6 and most of the biological effects of IL-13 have been linked to STAT6 activation. IL-13 inhibits the production of pro-inflammatory cytokines but can up-regulate the synthesis of IL-12 in dendritic cells and macrophages. IL-13 is typically associated with allergic inflammation, including asthma. It induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia, and glycoprotein hypersecretion, which all contribute to airway obstruction. (Ref.: Marone, G., et al (2019). Front. Pharmacol. 10; 1387; Van Der Pouw Kraan, TCTM., et al. (1998). Clin. Exp. Immunol. 111(1): 129-135).

特異性

Clone 6D11.8.11 is a mouse monoclonal antibody that detects human Interleukin-13.

免疫原

Recombinant fragment corresponding to 114 amino acids (aa 33-146) from human Interleukin-13 expressed in CHO cells.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting with human recombinant IL-13.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected human recombinant IL-13.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-13 in human testis tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-13, clone 6D11.8.11, Cat. No. MABF2287, is a mouse monoclonal antibody that detects Interleukin-13 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

保管および安定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable