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製品名
Hygromycin B, Streptomyces sp., Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells.
品質水準
アッセイ
>85% (HPLC)
フォーム
liquid
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
色
amber to brown
輸送温度
ambient
保管温度
2-8°C
InChI
1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
InChI Key
GRRNUXAQVGOGFE-KPBUCVLVSA-N
詳細
Bioassay: 350-450 units/mg Hygromycin B. 106 units is equivalent to ~900 mg.
Hygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template (Dean, N., Gonzalez, A., et al., Rao, S.N., et al.). Hygromycin B has been used to select mutants in a wide variety of cells including bacteria (Bilang, R., et al., Salauze, D., et al.), protozoans (Lee, M.G-S., and Van der Ploeg, L.H.T.), yeast (Perlin, D.S., et al.), fungi (Cullen, D., et al., Kronstad, J.W., et al., Egelhoff, T.T., et al., Leslie, J.F., and Dickman, M.B., Bulte, L., and Bennoun, P.), plants (Dean, N., Damm, B., et al., Rikkerink, E. H., et al., Sugimoto, K., et al.), and mammalian cells (Crespi, C.L., et al., Giordano, T.J., and McAllister, W.T., Hubbard, S. C., et al.).
Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).
The analytical data listed below will vary from lot to lot.
Bioassay: 350-450 U/mg
Concentration: 444,600 U/ml; 394 mg Hygromycin B/ml
Density: 1140-1155 mg/ml
% solids: 34.6%
Fill volumes:
100,000 U = 225 µl
1,000,000 U = 2.25 ml
10,000,000 U = 22.5 ml
250,000 U = 562 µl
5,000,000 U = 11.25 ml
Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).
The analytical data listed below will vary from lot to lot.
Bioassay: 350-450 U/mg
Concentration: 444,600 U/ml; 394 mg Hygromycin B/ml
Density: 1140-1155 mg/ml
% solids: 34.6%
Fill volumes:
100,000 U = 225 µl
1,000,000 U = 2.25 ml
10,000,000 U = 22.5 ml
250,000 U = 562 µl
5,000,000 U = 11.25 ml
Supplied with a directional insert. Note: 1 KU = 1000 units; 1 MU = 1,000,000 units.
Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent.
生物化学的／生理学的作用
Primary Target
growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
Secondary Target
protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA
protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA
The activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
警告
Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)
再構成
Aqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (<2 mg/ml) are stable for up to 1 month at 4°C.
Note: This product is highly toxic. Read enclosed MSDS before handling.
Note: This product is highly toxic. Read enclosed MSDS before handling.
その他情報
Dean, N. 1995. Proc. Natl. Acad. Sci. USA92, 1287.
Hamada, W., et al. 1994. Curr. Genetics 26, 251.
Hubbard, S. C., et al. 1994. J. Biol. Chem.269, 3717.
Rikkerink, E. H., et al. 1994. Current Genetics25, 202.
Sugimoto, K., et al. 1994. Plant J.5, 863.
Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol.66, 2731.
Gaken, J., et al. 1992. Biotechniques13, 32.
Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
Ma, H., et al. 1992. Gene117, 161.
MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol.276, 67.
Norman, J.A., et al. 1992. Mol. Pharmacol.41, 53.
Bilang, R., et al. 1991. Gene100, 247.
Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA88, 10558.
Lee, M.G-S., and Van der Ploeg, L. 1991. Gene105, 255.
Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol.57, 1423.
MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother.35, 2125.
Zhou, J., et al. 1991. Gene107, 307.
Bulte, L., and Bennoun, P. 1990. Current Genetics18, 155.
Giordano, T.J., and McAllister, W.T. 1990. Gene88, 285.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother.24, 1915.
Carrasco, L., et al. 1989. Pharmacol. Ther.9, 311.
Crespi, C.L., et al. 1989. Carcinogenesis10, 295.
Damm, B., et al. 1989. Mol. Gen. Genetics217, 6.
Egelhoff, T.T., et al. 1989. Mol. Cell. Biol.9, 1965.
Kronstad, J.W., et al. 1989. Gene79, 97.
Perlin, D.S., et al. 1988. J. Biol. Chem.263, 118.
Cullen, D., et al. 1987. Gene57, 21.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459.
Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun.117, 6.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.
Hamada, W., et al. 1994. Curr. Genetics 26, 251.
Hubbard, S. C., et al. 1994. J. Biol. Chem.269, 3717.
Rikkerink, E. H., et al. 1994. Current Genetics25, 202.
Sugimoto, K., et al. 1994. Plant J.5, 863.
Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol.66, 2731.
Gaken, J., et al. 1992. Biotechniques13, 32.
Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
Ma, H., et al. 1992. Gene117, 161.
MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol.276, 67.
Norman, J.A., et al. 1992. Mol. Pharmacol.41, 53.
Bilang, R., et al. 1991. Gene100, 247.
Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA88, 10558.
Lee, M.G-S., and Van der Ploeg, L. 1991. Gene105, 255.
Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol.57, 1423.
MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother.35, 2125.
Zhou, J., et al. 1991. Gene107, 307.
Bulte, L., and Bennoun, P. 1990. Current Genetics18, 155.
Giordano, T.J., and McAllister, W.T. 1990. Gene88, 285.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother.24, 1915.
Carrasco, L., et al. 1989. Pharmacol. Ther.9, 311.
Crespi, C.L., et al. 1989. Carcinogenesis10, 295.
Damm, B., et al. 1989. Mol. Gen. Genetics217, 6.
Egelhoff, T.T., et al. 1989. Mol. Cell. Biol.9, 1965.
Kronstad, J.W., et al. 1989. Gene79, 97.
Perlin, D.S., et al. 1988. J. Biol. Chem.263, 118.
Cullen, D., et al. 1987. Gene57, 21.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459.
Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun.117, 6.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
保管分類コード
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
400051-100MU:
400051-50MU:
400051-1MU:
400051-5MU:
400051-VAR:
400051-10MU:
400051-100KU:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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