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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
9D10, monoclonal
交差性
human, mouse
テクニック
ChIP: suitable (ChIP-seq)
western blot: suitable
アイソタイプ
IgG2bκ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... IKZF3(22806)
詳細
Aiolos is a zinc finger DNA binding transcription factor important in lymphocyte differentiation and development. Aiolos plays an essential role in the regulation of B-cell differentiation including, proliferation and maturation to plasma cells. Aiolos also works as a cofactor in gene suppression via its interaction with the transcription factor FOXP3 in regulatory T cells as well, and Aiolos is involved in various chromatin functions too through its interaction with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A. Aiolos is localized in the nucleus primarily but can be found in the cytoplasm depending upon the cellular situation. Aiolos is expressed in leukocytes and found in high concentration in spleen and thymus and has multiple isoforms. Activity is regulated via phosphorylation.
免疫原
Recombinant protein corresponding to human Aiolos.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
転写因子
転写因子
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).
ChIP-seq: A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in an HL-60 cell lysate
ChIP-seq: A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in an HL-60 cell lysate
This Anti-Aiolos Antibody, clone 9D10 is validated for use in Western Blotting and ChIP-seq and Chromatin Immunoprecipitation (ChIP) for the detection of Aiolos.
品質
Evaluated by Western Blotting in a Jurkat cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in 10 µg of a Jurkat cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in 10 µg of a Jurkat cell lysate.
ターゲットの説明
~ 58-70 kDa observed
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABE911:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Gregory Lazarian et al.
Cancer cell, 39(3), 380-393 (2021-03-11)
Hotspot mutation of IKZF3 (IKZF3-L162R) has been identified as a putative driver of chronic lymphocytic leukemia (CLL), but its function remains unknown. Here, we demonstrate its driving role in CLL through a B cell-restricted conditional knockin mouse model. Mutant Ikzf3
Hye Sun Kuehn et al.
The Journal of experimental medicine, 218(12) (2021-10-26)
AIOLOS/IKZF3 is a member of the IKAROS family of transcription factors. IKAROS/IKZF1 mutations have been previously associated with different forms of primary immunodeficiency. Here we describe a novel combined immunodeficiency due to an IKZF3 mutation in a family presenting with
Jiangwen Zhang et al.
Nature immunology, 13(1), 86-94 (2011-11-15)
Cell fate depends on the interplay between chromatin regulators and transcription factors. Here we show that activity of the Mi-2β nucleosome-remodeling and histone-deacetylase (NuRD) complex was controlled by the Ikaros family of lymphoid lineage-determining proteins. Ikaros, an integral component of
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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