MABE644

Anti-Cre Recombinase Antibody, clone 5B4.1

clone 5B4.1, from mouse

• 別名: Recombinase cre
• UNSPSCコード: 12352203
• eCl@ss: 32160702

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 5B4.1, monoclonal
• 交差性: virus
• 交差性（ホモロジーによる予測）: all
• テクニック: western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: YP_006472
• UniProtアクセッション番号: P06956
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: Escherichia coli ... Cre(2777477)

詳細

詳細

Recombinase cre (UniProt P06956) is encoded by the cre gene (Gene ID 2777477) of bacteriophage P1. Cre (Causes recombination with Cyclization recombinase) is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34-bp DNA sequence motif called loxP (locus of X-over P1) found in P1 DNA. The loxP site is composed of a variable asymmetric 8-bp sequence in between two sets of 13-bp palindromic sequences (ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT). Two cre recombinase proteins, each binds to one of the two 13-bp palindromic sequences at a loxP site, form a dimer. This dimer then binds to a second dimer on another loxP site to form a tetramer. The two sites joined by the tetramer are parallel in orientation and the double stranded DNA is cut at both loxP sites. The strands are then rejoined with DNA ligase. Because the Cre gene and loxP sites are not native to most species, they are often introduced into target cells or tissues and employed as a means to precisely control the expression of genes both in cultured cells in vitro and in animals vivo.

特異性

Clone 5B4.1 detects an epitope outside the N-terminal region.

免疫原

GST-tagged recombinant Cre lacking the N-terminal region sequence.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Anti-Cre Recombinase Antibody, clone 5B4.1, Cat. No. MABE644, is a highly specific mouse monoclonal antibody that targets Cre recombinase and has been tested in Western Blotting.

品質

Evaluated by Western Blotting in recombinant Cre.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected 250 ng of recombinant Cre (with added N- and C-terminal tag sequences).

ターゲットの説明

38.54 kDa calculated.

関連事項

Replaces: MABE3120

物理的形状

Protein G purified.

Format: Purified

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable