MABE1919

Anti-TNRC6ABC Antibody, clone 7A9

clone 7A9, from rat

• 別名: Trinucleotide repeat-containing gene 6A, 6B, and 6C, CAG repeat protein 26, EMSY interactor protein, GW182 autoantigen, Protein GW1, Glycine-tryptophan protein of 182 kDa
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rat
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 7A9, monoclonal
• 化学種の反応性: human
• 包装: antibody small pack of 25 μg
• テクニック: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_001020014; NP_001136112; NP_001317449
• UniProtアクセッション番号: Q8NDV7; Q9HCJ0; Q9UPQ9
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TNRC6B(23112)

詳細

詳細

Trinucleotide repeat-containing gene 6A, 6B, and 6C proteins (UniProt: Q8NDV7; Q9UPQ9; Q9HCJ0) are encoded by the TNRC6A, TNRC6B, and TNRC6C genes (Gene ID: 27327, 23112, and 57690), respectively in human. TNRC6 proteins are ubiquitously distributed and play a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). TNRC6 proteins are required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. They also serve as a scaffolding proteins and associate with argonaute proteins bound to partially complementary mRNAs. They can simultaneously recruit CCR4-NOT and PAN deadenylase complexes. TNRC6 proteins are predominantly found in the cytoplasm under steady state conditions, however, they are also found in the nucleus and their nuclear import is mediated via the importin pathways. TNRC6A is shown to posses both nuclear localization (NLS) as well a nuclear export signal (NES). In case of mutated NES, TNRC6A is shown to be retained in the nucleus. TNRC6 proteins contain RRM domains (aa 1781-1853; 1648-1720; and 1563-1632 for TNRC6A, 6B, and 6C, respectively). Clone 7A9 recognizes all three TNRC6 proteins. (Ref.: Schraivogel, D., et al. (2015). Nucleic Acids Res. 43(15); 7447-7461).

特異性

Clone 7A9 is a rat monoclonal antibody that detects human Trinucleotide repeat-containing gene 6A, 6B, and 6C proteins. It target epitopes with in RRM domains.

免疫原

Recombinant RRM domain regions from human TNRC6A, TNRC6B, and TNRC6C.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Anti-TNRC6ABC, clone 7A9, Cat. No. MABE1919, is a highly specific rat monoclonal antibody that targets TNRC6 and has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Immunofluorescence Analysis: A representative lot detected TNRC6ABC in Immunofluorescence applications (Schraivogel, D., et. al. (2015). Nucleic Acids Res. 43(15):7447-61).

Immunoprecipitation Analysis: A representative lot immunoprecipitated TNRC6ABC in Immunoprecipitation applications (Quevillon Huberdeau, M., et. al. (2017). EMBO J. 36(14):2088-2106).

品質

Evaluated by Western Blotting in Induced Stably transfected HEK293 cell lysates.

Western Blotting Analysis: 4 µg/mL of this antibody detected TNRC6 in lysate from stably induced HEK 293 cells transfected with TNRC6A, but not in uninduced HEK 293 cells.

ターゲットの説明

~175-210 kDa observed; 210.30,194.00, 175.96 kDa for TNRC6A, 6B, and 6C, respectively. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable