MABE143

Anti-Ago1 Antibody, clone 4G7-E12

clone 4G7-E12, from mouse

• 別名: hAgo1, Argonaute1, eIF-2C 1, eIF2C 1, Q99, GERP95, DKFZp686M13167
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 4G7-E12, monoclonal
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_036331
• UniProtアクセッション番号: Q9UL18
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... AGO1(26523)

詳細

詳細

Ago1 (Eukaryotic translation initiation factor 2C 1) belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as a cell cycle control protein through its regulation of cytokinesis and its related cell cycle checkpoints, which occur downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage, but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.

免疫原

GST-tagged recombinant protein corresponding to human Ago1.

アプリケーション

Anti-Ago1 Antibody, clone 4G7-E12 is a Mouse Monoclonal Antibody for detection of Ago1 also known as hAgo1, Argonaute1, eIF-2C 1 & has been validated in WB.

Immunoprecipitation: A representative lot of this antibody was used for immunoprecipitation by an independent laboratory (see Azuma-Mukai A. et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).

品質

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Ago1 in HeLa cell lysate.

ターゲットの説明

~97 kDa observed.

物理的形状

Format: Purified

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable