由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
180, monoclonal
化学種の反応性
mouse
テクニック
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG2aκ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... Spo11(26972)
詳細
Meiotic recombination protein SPO11 (UniProt Q9WTK8) is encoded by the Spo11 gene (Gene ID 26972) in murine species. SPO11 is a widely conserved protein that is involved in meiotic recombination and mediates DNA cleavage that forms the double-strand breaks that initiate meiotic recombination. Active site of SPO11 contains a tyrosine, which ligates and dissociates with DNA to promote break formation. One SPO11 protein is involved per strand of DNA, hence, two SPO11 proteins are involved in each double stranded break event. High levels of SPO11 are found only in the testis where expression is restricted primarily to meiotic germ cells and it is not expressed in spermatogonia. Very low levels of SPO11 have been reported in thymus, brain, and oocytes of embryonic ovary, but it is not detected in adult ovary. Mouse and human SPO11 exhibit about 82% sequence homology. Ref.: Keeney, S., et al. (1997). Cell 375-384.
特異性
Clone 180 detected SPO11alpha and SPO11beta in wild-type, but not Spo11-knockout mouse testes lysates by IP-Western blotting.
免疫原
Recombinant mouse SPO11 with N-terminal S-tag and C-terminal His-tag.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Anti-SPO11, clone 180, Cat. No. MABE1167, is a highly specific mouse monoclonal antibody that targets SPO11 and has been tested in and Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Immunoprecipitation Analysis: 3 µg from a representative lot immunoprecipitated SPO11alpha and SPO11beta from wild-type, but not Spo11-knockout mouse testes lysates (Courtesy of the Keeney Lab, MSKCC, New York, U.S.A.).
Western Blotting Analysis: A representative lot detected SPO11alpha and SPO11beta in immunoprecipitate from wild-type, but not SPO-knockout, mouse testes using the same antibody (Courtesy of the Keeney Lab, MSKCC, New York, U.S.A.).
Immunocytochemistry Analysis: A representative lot immunostained SPO11 in meiotic prophase I leptotene cells by fluorescence immunocytochemistry staining of chromosome spreads from isolated mouse spermatocytes hypotonically swollen, fixed with 1% formaldehyde and permeabilized with 0.1% Triton X-100 (Alpatov, R., et al. (2014). Cell. 157(4):869-881).
Immunoprecipitation Analysis: A representative lot immunoprecipitated SPO11alpha and SPO11beta as well as SPO11-oligo complexes from mouse testes lysates. Much elevated and greatly reduced SPO11-oligo complexes were found in Atm-/- and Dmc-/- testes IP, respectively, when comparing to wild-type testes IP (Lange, J., et al. (2011). Nature. 479(7372):237-240).
Western Blotting Analysis: Representative lots detected SPO11alpha and SPO11beta in immunoprecipitates obtained with the same antibody from mouse testes. Much reduced SPO11 was found in Atm-/- and Dmc-/- testes IPs due to meiotic arrest at prophase I (Biswas, U., et al. (2013). PLoS Genet. 9(12):e1003985; Lange, J., et al. (2011). Nature. 479(7372):237-240).
Western Blotting Analysis: A representative lot detected SPO11alpha and SPO11beta in immunoprecipitate from wild-type, but not SPO-knockout, mouse testes using the same antibody (Courtesy of the Keeney Lab, MSKCC, New York, U.S.A.).
Immunocytochemistry Analysis: A representative lot immunostained SPO11 in meiotic prophase I leptotene cells by fluorescence immunocytochemistry staining of chromosome spreads from isolated mouse spermatocytes hypotonically swollen, fixed with 1% formaldehyde and permeabilized with 0.1% Triton X-100 (Alpatov, R., et al. (2014). Cell. 157(4):869-881).
Immunoprecipitation Analysis: A representative lot immunoprecipitated SPO11alpha and SPO11beta as well as SPO11-oligo complexes from mouse testes lysates. Much elevated and greatly reduced SPO11-oligo complexes were found in Atm-/- and Dmc-/- testes IP, respectively, when comparing to wild-type testes IP (Lange, J., et al. (2011). Nature. 479(7372):237-240).
Western Blotting Analysis: Representative lots detected SPO11alpha and SPO11beta in immunoprecipitates obtained with the same antibody from mouse testes. Much reduced SPO11 was found in Atm-/- and Dmc-/- testes IPs due to meiotic arrest at prophase I (Biswas, U., et al. (2013). PLoS Genet. 9(12):e1003985; Lange, J., et al. (2011). Nature. 479(7372):237-240).
品質
Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2aκ.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2aκ.
ターゲットの説明
~44/40 kDa (SPO11beta/SPO11alpha) observed. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Protein G purified.
Format: Purified
Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABE1167:
試験成績書（COA）
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