MABE1132

Anti-TET2 Antibody, clone 9F7

clone 9F7, from rat

• 別名: Methylcytosine dioxygenase TET2, Protein Ayu17-449
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 9F7, monoclonal
• 化学種の反応性: mouse
• テクニック: ELISA: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_001035490
• UniProtアクセッション番号: Q4JK59
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Tet2(214133)

詳細

詳細

Methylcytosine dioxygenase TET2 (EC 1.14.11.n2; UniProt Q4JK59; also known as Protein Ayu17-449) is encoded by the Tet2 (also known as Kiaa1546) gene (Gene ID 214133) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.

特異性

Clone 9F7 detected exogenously expressed TET2, but not TET1 or TET3, GST fusion (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812). Epitope region is present in all three murine TET2 spliced isoforms reported by UniProt (Q4JK59).

免疫原

Epitope: Region between DNA-binding and 2-oxoglutarate-binding domains.

His-tagged recombinant fragment from the region between DNA-binding and 2-oxoglutarate-binding domains of mouse TET2.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
エピジェネティクス

Anti-TET2 Antibody, clone 9F7 is an antibody against TET2 for use in Immunocytochemistry, Western Blotting, Immunoprecipitation, ELISA.

Western Blotting Analysis: A representative lot detected exogenously expressed TET2, but not TET1 or TET3, GST fusion in lysates from respective HEK293T transfectants (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous TET2 from murine embryonic stem cell (mESC) lysate. Subsequent LC-MS/MS analysis detected the presence of co-immunoprecipitated glycosyltransferase OGT (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Analysis: Clone 9F7 hybridoma culture supernatant was confirmed for its immunoreactivity against His-tagged murine TET2 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).

品質

Evaluated by Immunocytochemistry in mouse mouse ESCs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected TET2 in mouse embryonic stem cells (ESCs).

ターゲットの説明

212.1 kDa (isoform 1), 68.06 kDa (isoform 2), 64.50 kDa (isoform 3) calculated.

物理的形状

Protein G purified.

Format: Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable