MABD396

Anti-Sortilin Antibody, clone 4E3.1

clone 4E3.1, from mouse

• 別名: Sortilin, 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.75

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 4E3.1, monoclonal
• 化学種の反応性: human, mouse
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_002950
• UniProtアクセッション番号: Q99523
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SORT1(6272)

詳細

詳細

Sortilin (UniProt Q99523; also known as 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3) is encoded by the SORT1 (also known as LDLCQ6, NT3) gene (Gene ID 6272) in human. Sortlin belongs to the a family of sorting receptors characterized by the Vps10p domain that creates a tunnel cavity with its ten-bladed β-propeller for the binding of soluble ligands. The family members also contain a short cytoplasmic tail that harbors recognition motifs for subcellular sorting adaptor proteins. Sortilin is reproted to bind PCSK9 in the trans-Golgi network with high-affinity in HepG2 cells. Sort1 knockout mice exhibit reduced PCSK9 plasma concentration and altered PCSK9 subcellular localization in hepatocytes. Sortilin is also found to colocalize with APP in neurites and facilitates APP processing toward the production of sAPPα, possibly by optimizing APP as a substrate for α-secretase cleavage. Sortilin is initially produced with a signal peptide (a.a. 1-33) and a propeptide (a.a. 34-77) sequence, the removal of which yields the mature receptor (a.a. 78-831) with a large luminal/extracellular (a.a. 78-555) portion, a transmembrane region (a.a. 756-778), and a short cytoplasmic tail (a.a.779-831). Similar to the other two family members (MPRs and SorLA), sortilin resides both on the membrane of intracellular vesicles and on the plasma membrane. Altered sortilin expressions due to SORT1 genetic variations affect serum levels of low density lipoprotein cholesterol (LDL-C) and contribute to the LDL-C level quantitative trait locus 6 (LDLCQ6) and susceptibility to myocardial infarction.

特異性

Expected to react with both spliced isoforms reported by UniProt (Q99523).

免疫原

Epitope: C-terminal region.

GST-tagged recombinant human Sortilin C-terminal fragment.

アプリケーション

Research Category
幹細胞研究

Research Sub Category
小胞輸送

Anti-Sortilin Antibody, clone 4E3.1 is an antibody against Sortilin for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: An 1:250 dilution of this antibody from a representative lot detected Sortilin in human cerebral cortex, colon, and kidney tissue sections.

品質

Evaluated by Western Blotting in E13-14 embryonic mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Sortilin in 10 µg of E13-14 embryonic mouse brain tissue lysate.

ターゲットの説明

~92 kDa observed. Target band size appears larger than the calculated molecular weights of 83.86 kDa (isoform 1) and 69.17 kDa (isoform 2) due to glycosylation. Uncharacterized band(s) may appear in some lysates.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable