MABC535

Anti-BIRC2/IAP-2, clone 1E1-1-10 Antibody

clone 1E1-1-10, from rat

• 別名: Baculoviral IAP repeat-containing protein 2, Inhibitor of apoptosis protein 2, IAP-2, mIAP-2, mIAP2, cIAP1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 1E1-1-10, monoclonal
• 化学種の反応性: mouse, human
• テクニック: western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_031491
• UniProtアクセッション番号: Q13490; Q62210
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... BIRC2(329)

詳細

詳細

BRC2/IAP-2, also known as IAP homolog B, Inhibitor of apoptosis protein 2 and Baculoviral IAP repeat-containing protein 2, and encoded by the gene BIRC2/API1/IAP2/MIHB/ RNF48, is a multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. BIRC2/IAP-2 also acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical pathways. Additionally, BIRC2/IAP-2 acts as an important regulator of innate immune signaling, and finally, BIRC2/IAP-2 plays an important role in the modulation of the cell cycle. BIRC2/IAP-21 is localized to nucleus except during apoptotic events. BIRC2/IAP-2 is expressed in fetal and adult tissues with a strong emphasis found in adult skeletal muscle, thymus, testis, ovary and pancreas with low or absent expression in blood and brain.

免疫原

Epitope: This antibody recognizes the BIR 2 repeat region of BIRC2/IAP-2.

Linear peptide corresponding to the BIR 2 repeat region of mouse BIRC2/IAP-2.

アプリケーション

Research Category
アポトーシス及び癌

Research Sub Category
アポトーシス-追加

Anti-BIRC2/IAP-2 antibody, clone 1E1-1-10 is an antibody against BIRC2/IAP-2 for use in western blotting.

Western Blotting Analysis: 2 µg/mL from a representative lot detected BIRC2/IAP-2 in C2C12 cell lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in HeLa and Raji cell lysate (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.).

Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in MEF cell lysate (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.).

Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in stable cell lines expressing EYFP cIAP1 RING, or EYFP cIAP1 RING (Silke, J., et al. (2005). Proc Natl Acad Sci USA. 102(45):16182-16187.).

品質

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected BIRC2/IAP-2 in 10 µg of NIH/3T3 cell lysate.

ターゲットの説明

~65 kDa observed. The calculated molecular weight of this protein is 70 kDa; however, this protein has been observed at lower molecular weights (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.; Silke, J., et al. (2005). Proc Natl Acad Sci USA. 102(45):16182-16187.).

物理的形状

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable