MABC1092

Anti-ASPP2 (TP53BP2) Antibody, clone DX54.10

clone DX54.10, from mouse

• 別名: Apoptosis-stimulating of p53 protein 2, Bcl2-binding protein, Bbp, Renal carcinoma antigen NY-REN-51, Tumor suppressor p53-binding protein 2, 53BP2, p53-binding protein 2, p53BP2
• UNSPSCコード: 12352203
• eCl@ss: 32160702

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: DX54.10, monoclonal
• 交差性: human
• 包装: antibody small pack of 25 μL
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• UniProtアクセッション番号: Q13625
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TP53BP2(7159)

詳細

詳細

Apoptosis-stimulating of p53 protein 2 (UniProt: Q13625; also known as Bcl2-binding protein, Bbp, Renal carcinoma antigen NY-REN-51, Tumor suppressor p53-binding protein 2, 53BP2, p53-binding protein 2, p53BP2) is encoded by the TP53BP2 (also known as ASPP2, BBP) gene (Gene ID: 7159) in human. 53BP2 plays a central role in regulation of apoptosis and cell growth. It is widely expressed and its expression is observed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte 53BP2 is induced following DNA damage by UV radiation. It is down-regulated by wild-type p53/TP53, but not by mutant p53/TP53. It regulates p53/TP53 action by enhancing the DNA binding and transactivation function of TP53 on the promoters of pro-apoptotic genes in vivo. 53BP2 is shown to inhibit the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. 53BP2 contains four ankyrin repeats and one SH3 domain are required for a specific interactions with TP53. The 53BP2 binding site on the p53 core domain consists of evolutionarily conserved regions that are frequently mutated in human cancers. Lower level of 53BP2 expression is observed in breast carcinomas expressing a wild-type TP53 protein.

特異性

Clone DX54.10 detects Apotosis -stimulating of p53 protein 2 (TP53BP2) in human cells. It targets an epitope with in 438 amino acids from the C-terminal half.

免疫原

Epitope: C-terminus

GST-tagged recombinant fragment corresponding to 438 amino acids from the C-terminal half of human Apoptosis-stimulating of p53 protein 2 (TP53BP2).

アプリケーション

Research Category
アポトーシス及び癌

Anti-ASPP2 (TP53BP2), clone DX54.10, Cat. No. MABC1092, is a mouse monoclonal antibody that detects Apoptosis-stimulating of p53 protein 2 (ASPP2) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunoprecipitation Analysis: A representative lot detected ASPP2 (TP53BP2) in Immunoprecipitation applications (Samuels-Lev, Y., et. al. (2001). Mol Cell. 8(4):781-94).

Immunohistochemistry Analysis: A representative lot detected ASPP2 (TP53BP2) in Immunohistochemistry applications (Wang, Y., et. al. (2014). Nat Cell Biol. 16(11):1092-104).

Immunocytochemistry Analysis: A representative lot detected ASPP2 (TP53BP2) in Immunocytohemistry applications (Wang, Y., et. al. (2013). Cell Death Differ. 20(4):525-34; Wang, Y., et. al. (2014). Nat Cell Biol. 16(11):1092-104).

Western Blotting Analysis: A representative lot detected ASPP2 (TP53BP2) in Western Blotting applications (Wang, Y., et. al. (2013). Cell Death Differ. 20(4):525-34; Samuels-Lev, Y., et. al. (2001). Mol Cell. 8(4):781-94).

品質

Evaluated by Western Blotting in U2OS-iASPP and U2OS RISC-free cell lysates.

Western Blotting Analysis: A 1:125 dilution of this antibody detected ASPP2 in lysates of U2OS cells transfected with siRNA to knock down iASPP and in RISC-free U2OS cells.

ターゲットの説明

~170 kDa observed; 125.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable