MAB8151-I

Anti-West Nile Virus/Kunjin Antibody, Envelope Antibody, clone 3.91D

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 3.91D, monoclonal
• 分子量: calculated mol wt 31.89 kDa; observed mol wt ~50 kDa
• 精製方法: using protein G
• 化学種の反応性: virus
• 包装: antibody small pack of 100 μL
• テクニック: ELISA: suitable; immunofluorescence: suitable; western blot: suitable
• アイソタイプ: IgG3
• エピトープ配列: N-terminal half
• タンパク質IDアクセッション番号: NP_776015.1
• UniProtアクセッション番号: P14335
• 輸送温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: vaccinia virus ... poly> POLY(912267)

詳細

詳細

Genome polyprotein (UniProt: P14335) is encoded by the POLY gene (Gene ID: 912267) in West Nile virus. West Nile virus (WNV) is a mosquito-borne positive-sense RNA virus of the Flavivirus genus within the Flaviviridae family. WNV genome encodes for three structural domains known as C, prM, and E and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). These are translated as a single polypeptide, which is subsequently cleaved by viral and cellular proteases. The envelope glycoprotein (E) undergoes a conformational rearrangement triggered by low pH that results in a class II fusion event required for viral entry. The E protein (aa 291-791) contains a three-domain structure where domain I is an eight-stranded β-barrel in the center portion of the protein (aa 1-51, 134-195, and 284-297). The DII domain consists of 170 residues inserted into DI (aa 52-133 and 196-283). At the distal end of DII domain is a fusion loop (aa 98-110) and a conserved glycine-rich, hydrophobic sequence, which is important for flaviviral fusion. The third domain, DIII, is an Ig-like domain with seven β-strand and has been implicated in receptor binding. The E protein also has a single N-linked glycosylation site at Asn 154, located within the ectodomain of prM domain and is cleaved by cellular enzymes during viral particle maturation. Clone E60 specifically binds to the DII fusion loop and displays limited neutralizing activity and even at higher doses neutralization activity is shown to be between 26 and 68%. (Ref.: Martín-Acebes MA, et al. (2012). World J Virol. 1(2); 51-70).

特異性

Clone 3.91D is a mouse monoclonal antibody that detects West Nile virus envelope protein. It targets an epitope within the N-terminal half of polyprotein.

免疫原

West Nile Virus (WNV; subtype KUNV) isolate OR393.

アプリケーション

Qualtiy Control Testing

Evaluated by Western Blotting with recombinant West Nile Virus envelope protein.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant West Nile Virus envelope protein.

Tested Applications

Western Blotting Analysis: A representative lot detected West Nile Virus/Kunjin, Envelope protein in Western Blotting applications (Maeda, A., et al. (2009). Virus Res.;144(1-2):35-43; Saiyasombat, R., et al. (2014). Virol J.;11:150; Blitvich, B.J., et al. (2016). Am J Trop Med Hyg.;95(5):1185-1191).

ELISA Analysis: Various dilutions of this antibody detected recombinant West Nile Virus/Kunjin, Envelope protein.

Immunofluorescence Analysis: A representative lot detected West Nile Virus/Kunjin, Envelope protein in Immunofluorescence applications (Maeda, A., et al. (2009). Virus Res.;144(1-2):35-43; Osorio, J.E., et al. (2012). Am J Trop Med Hyg.;87(3):565-72).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-West Nile Virus/Kunjin, Envelope, clone 3.91D, Cat. No. MAB8151-I, is a mouse monoclonal antibody that detects envelope protein in West Nile virus and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable