MAB1926-I

Anti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11

clone P1H11, from mouse

• 別名: FN, Cold-insoluble globulin, CIG
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: P1H11, monoclonal
• 化学種の反応性: human, porcine, mouse
• 包装: antibody small pack of 25 μL
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NM_002026
• UniProtアクセッション番号: P02751
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FN1(2335) ; mouse ... Fn1(14268)

詳細

詳細

Fibronectin (UniProt: P02751; also known as FN, Cold-insoluble globulin, CIG) is encoded by the FN1 (also known as FN) gene (Gene ID: 2335) in human. Fibronectin is are involved various biological activities, including cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. It is essential for osteoblast mineralization and participates in the regulation of type I collagen deposition by osteoblasts. Fibronectin is usually present as homodimer of ~250 kDa subunits where subunits are connected by two disulfide bonds at the C-terminal region. Each monomer contains three repeating units, which are designated as type I, II, and III repeats containing about 40, 60, and 90 amino acids, respectively. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes and the cellular fibronectin (dimeric or cross-linked multimeric forms) is produced by fibroblasts, epithelial and other cell types. Cellular fibronectin is deposited as fibrils in the extracellular matrix. Fibronectin is recognized by integrins alpha5beta1 and alphaVbeta3. The primary sequence motif of fibronectin for integrin binding is a tripeptide, Arg-Gly-Asp (RGD). Mutations in FN gene are known to cause glomerulopathy that is characterized by proteinuria, microscopic hematuria, and hypertension, which can lead to end-stage renal failure.

特異性

Clone P1H11 detects fibronectin in human.

免疫原

Human Plasma Fibronectin.

アプリケーション

Research Category
細胞骨格

Anti-Fibronectin, cell binding domain, clone P1H11, Cat. No. MAB1926-I is a mouse monoclonal antibody that detects human fibronectin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Fibronectin in human kidney and human cerebellum tissues.

Western Blotting Analysis: A representative lot detected Fibronectin in Western Blotting applications (Zhang, H., et. al. (2010). Am J Physiol Renal Physiol. 299(1):F91-8).

Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Mardilovich, A., et. al. (2006). Langmuir. 22(7):3259-64).

Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Hofanson, D.M., et. al. (2010). Biomaterials. 31(26):6730-7).

Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Waterman, R.S., et. al. (2010). PLoS One. 5(4):e10088).

Immunohistochemistry Analysis: A representative lot detected Fibronectin in Immunohistochemistry applications (Carlos, C.P., et. al. (2014). PLoS One. 9(7):e103660).

Western Blotting Analysis: A representative lot detected Fibronectin in Western Blotting applications (Zhang, H., et. al. (2008). Am J Physiol Renal Physiol. 295(4):F1071-81).

品質

Evaluated by Western Blotting in human thymus tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Fibronectin in 10 µg of human thymus tissue lysate.

ターゲットの説明

~270 kDa observed; 262.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable