MAB4625

Anti-Bcl-X Antibody, clone 2H12

clone 2H12, Chemicon^®, from mouse

• 別名: Bcl-2-like Protein
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 2H12, monoclonal
• 化学種の反応性: rat, pig, mouse, human
• メーカー／製品名: Chemicon^®
• テクニック: flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2a
• NCBIアクセッション番号: NM_001191.2; NM_138578.1
• UniProtアクセッション番号: Q07817
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... BCL2L1(598)

詳細

詳細

Bcl-X has two isoforms, Bcl-XL (long), a 241-aa protein, and Bcl-XS (short), a 178-aa protein lacking a 63 aa domain that is well conserved among members of the Bcl-2 family.

特異性

MAB4625 recognizes a protein of 27 kDa, identified as the Bcl-X protein. Reacts with both Bcl-XS and Bcl-XL.

免疫原

Synthetic peptide, aa 3-14 of human Bcl-X protein. This amino acid sequence is shared by human and murine Bcl-X protein.

アプリケーション

Research Category
アポトーシス及び癌

Research Sub Category
BCL2とその阻害

This Anti-Bcl-X Antibody, clone 2H12 is validated for use in FC, IF, IP, WB, IH for the detection of Bcl-X.

Wstern Blot: 1μg/mL (Hsu, 1997a; Hsu, 1997b)

Flow Cytometry

Immunofluorescence Immunoprecipitation: native protein (2ug/mg protein lysate).

Immunohistochemistry (frozen and paraffin): 2-4ug/mL for 30 minutes at room temperature.

Optimal working dilutions must be determined by end user.

物理的形状

Format: Purified

Protein A purified immunoglobulin in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

保管および安定性

Maintain at 2-8°C for up to 12 months.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable