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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
2D2, monoclonal
化学種の反応性
human
メーカー／製品名
Chemicon®
テクニック
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... BAX(581)
詳細
The previously assigned protein identifier Q07814 has been merged into Q07812. Full details can be found on the UniProt database.
特異性
Recognizes a 21 kD protein. Does not cross-react with Bcl-2a or Bcl-X. In contrast to Bcl-2a and Bcl-X, overexpression of Bax protein accelerates apoptosis.
免疫原
Epitope: N-terminus, aa3-16 hBax protein
Synthetic peptide corresponding to aa 3-16 (GSGEQPRGGGPTSS) of human bax. This amino acid sequence is not shared by mouse or rat Bax.
アプリケーション
Research Category
アポトーシス及び癌
アポトーシス及び癌
Research Sub Category
BCL2とその阻害
BCL2とその阻害
Anti-Bax Antibody, N-terminus, aa3-16 hBax protein, clone 2D2 is an antibody against Bax for use in IF, WB, IH(P).
Western blot: 1 μg/ml for 2 hours at RT (1:200 dilution)
Immunofluorescence
Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT)
Optimal working dilutions must be determined by end user.
Immunofluorescence
Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT)
Optimal working dilutions must be determined by end user.
関連事項
Replaces: 04-434
物理的形状
Format: Purified
Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
保管および安定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB4601:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Opa1-mediated cristae opening is Bax/Bak and BH3 dependent, required for apoptosis, and independent of Bak oligomerization.
Yamaguchi, R; Lartigue, L; Perkins, G; Scott, RT; Dixit, A; Kushnareva, Y; Kuwana et al.
Molecular Cell null
Sabrina L Spencer et al.
Nature, 459(7245), 428-432 (2009-04-14)
In microorganisms, noise in gene expression gives rise to cell-to-cell variability in protein concentrations. In mammalian cells, protein levels also vary and individual cells differ widely in their responsiveness to uniform physiological stimuli. In the case of apoptosis mediated by
Maotao Du et al.
Annals of translational medicine, 9(23), 1722-1722 (2022-01-25)
Aloe-emodin is reported as a potential cancer therapeutic agent due to its inhibition of the proliferation, migration, and invasion of cancer cells. This study aimed to confirm the effects of aloe-emodin on the progression of melanoma and identify the underlying
Suzanne Gaudet et al.
PLoS computational biology, 8(4), e1002482-e1002482 (2012-05-10)
Stochastic fluctuations in gene expression give rise to cell-to-cell variability in protein levels which can potentially cause variability in cellular phenotype. For TRAIL (TNF-related apoptosis-inducing ligand) variability manifests itself as dramatic differences in the time between ligand exposure and the
Marc Payton et al.
Molecular cancer therapeutics, 17(12), 2575-2585 (2018-09-30)
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