MAB3121

Anti-Bcl-XL and BclXs Antibody, clone 7B2.5

clone 7B2.5, Chemicon^®, from mouse

• 別名: Anti-BCL-XL
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 7B2.5, monoclonal
• application: FACS; IHC; IP; WB
• 化学種の反応性: human
• テクニック: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 1

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 7B2.5, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Chemicon^®
• テクニック: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG3
• NCBIアクセッション番号: NM_001191.2; NM_138578.1
• UniProtアクセッション番号: Q07817
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... BCL2L1(598)

詳細

特異性

Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems (Cohen, 1991). It plays a key role in tissue and organ development, as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment (Cohen & Duke, 1992). Bcl-2 and one of its homologues, Bcl-XL, protect cells from apoptosis (Nunez & Clarke, 1994; Cory, 1995), while other homologues of Bcl-2 such as Bax, Bad and Bak have been shown to enhance apoptosis (Oltvai, 1993; Farrow, 1995; Chittenden, 1995; Kiefer, 1995). Bcl-XL has been shown to block apoptosis which is induced by a variety of stimuli and, under certain conditions, offers greater protection against apoptosis than Bcl-2 (Boise, 1993; Gottschalk, 1994; Gonzalez-Garcia, 1995; Dole, 1995; Shimizu, 1995). In contrast, Bad and Bax inhibit the protective functions of Bcl-XL and Bcl-2, respectively. Although heterodimerization between Bcl-XL, Bad and Bcl-2/Bax was originally thought to be essential for the differential anti-apoptotic activity of Bcl-XL and Bcl-2 (Oltvai, 1993; Yin, 1994); other results suggest that the formation of heterodimers may not be necessary for this death-repressing activity Cheng, 1996; Gottschalk, 1996). This antibody recognizes Human Bcl-XL (Mr 29 kDa) and Bcl-Xs (Mr 21 kDa).

免疫原

Recombinant Bcl-XS.

アプリケーション

Research Category
アポトーシス及び癌

Research Sub Category
BCL2とその阻害

Detect Bcl-XL & BclXs using this Anti-Bcl-XL & BclXs Antibody, clone 7B2.5 validated for use in FC, IP, WB, IH.

Flow cytometry:
Immunoprecipitation (Cheng, 1996; Gottschalk, 1996)

Immunohistochemistry (Foreman, 1996; Wrone-Smith, 1995)

Western blotting: ( 0.5 μg/mL)

Optimal working dilutions must be determined by end user.

物理的形状

Format: Purified

Purified immunoglobulin - Ig fraction. Liquid in borate buffer saline, pH 8.2.

保管および安定性

Store at 2-8°C for up to 12 months.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H360FD
• 注意書き: P201 - P202 - P280 - P308 + P313 - P405 - P501
• 危険有害性の分類: Repr. 1B
• 保管分類コード: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable