MAB1992

Anti-Integrin α3β1 Antibody, clone M-KID2

clone M-KID2, Chemicon^®, from mouse

• 別名: VLA-3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: M-KID2, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; radioimmunoassay: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NM_002204.1; NM_005501.1
• UniProtアクセッション番号: P26006
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ITGA3(3675)

詳細

詳細

Integrins are a family of dimeric proteins that mediate cell-cell and extracellular matrix adhesion. Each VLA subtype (VLA-1, VLA-2, VL-3, VLA-4, VLA-5) is composed of a distinct alpha subunit noncovalently associated with a common beta subunit, as an alpha-beta dimer. Integrin VLA-3 (very late antigen, alpha-3/beta-1) is a cell surface receptor that binds to fibronectin, laminin, collagen type I and VI. VLA-3 is typically restricted in normal tissue but is widely expressed in many cultured tumor cells and correlates with the degree of invasiveness of malignant melanoma cells.

特異性

Recognizes an epitope related to the VLA-3 complex (alpha-3/beta-1 integrin). Specificity was determined by immunoprecipitation from a detergent extract of ³⁵S-methionine labeled KJ29 cells.

免疫原

Human renal carcinoma cell line (KJ29).

アプリケーション

Immunohistochemistry (works on Acetone-fixed paraffin-embedded tissues)

ELISA/RIA

Immunoprecipitation. When immunoprecipitated samples are run on SDS-PAGE under a) non-reducing conditions, two bands will be detected with an apparent molecular weight of 110 and 150kDa; and b) reducing (2% B-ME) conditions, a single molecular species of 130kDa is detected. Under reducing conditions both alpha3 and beta1 subunits run with the same apparent molecular weight.

Optimal working dilutions must be determined by end user.

Anti-Integrin α3β1 Antibody, clone M-KID2 detects level of Integrin α3β1 & has been published & validated for use in ELISA, IP, RIA, IC, IH(P).

物理的形状

Format: Purified

Immunoglobulin purified by Protein A. Purified immunoglobulin in PBS and 0.05% sodium azide as preservative.

保管および安定性

Maintain at 2°-8°C in undiluted aliquots for up to 12 months from date of receipt.

アナリシスノート

Control
Cell line: KJ29 renal carcinoma adherent cells.
Tissue: Kidney; antibody reacts with basal layer podocytes and basement membrane of distal tubules.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable