MAB13406

Anti-MMP-2 Antibody, pro and active form, clone VB3

clone VB3, Chemicon^®, from mouse

• 別名: Gelatinase A, 72 kDa Type IV Collagenase
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: VB3, monoclonal
• 交差性: human
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; immunofluorescence: suitable; western blot: suitable
• アイソタイプ: IgG1
• 適合性: not suitable for immunohistochemistry
• NCBIアクセッション番号: NM_004530.2
• UniProtアクセッション番号: P08253
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MMP2(4313)

詳細

特異性

The antibody recognizes proteins of 72kDa and 66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72 kDa collagenase IV or gelatinase A). Shows no cross-reactivity with pro and active forms of other MMPs. MMPs are proteolytic enzymes capable of degrading connective tissue components. Degradation of the extracellular matrix (ECM) is an essential step in tumor invasion and metastasis. MMPs each have different substrate specificities within the ECM and are important in its degradation. MMP-2 mainly degrades type IV collagen and denatured collagens. MMP activity is modulated by tissue inhibitors of metalloproteinases (TIMP). Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by TIMP have been implicated in the invasive potential of malignant tumors.Cellular Localization: cytoplasmic

免疫原

Epitope: pro and active form

Human native 72 kDa Gelatinase A.

アプリケーション

Research Category
細胞骨格

Research Sub Category
MMP及び組織メタロプロテアーゼ

This Anti-MMP-2 Antibody, pro & active form, clone VB3 is validated for use in ELISA, IF, WB for the detection of MMP-2.

Western blot: 1:200-1:400 for 2 hours at room temperature

ELISA

Immunofluorescence

Does not work for immunohistochemistry Optimal working dilutions must be determined by end user.

関連事項

Replaces: 04-1048

物理的形状

200 μg/mL. of antibody purified from ascites fluid by Protein G chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Format: Purified

保管および安定性

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

アナリシスノート

Control
POSITIVE CONTROL: Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta. Bladder, breast and ovarian carcinomas.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable