MABE1782

Anti-phospho-E2F1 (Ser375) Antibody, clone 71-10

clone 71-10, from rabbit

• 別名: Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

特性

• 由来生物: rabbit
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 71-10, monoclonal
• 交差性: human
• 包装: antibody small pack of 25 μL
• テクニック: ELISA: suitable; western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_005216.1
• UniProtアクセッション番号: Q01094
• ターゲットの翻訳後修飾: phosphorylation (pSer375/pSer375)
• 遺伝子情報: human ... E2F1(1869)

詳細

詳細

Transcription factor E2F1 (UniProt: Q01094; also known as E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1) is encoded by the E2F1 (also known as RBBP3) gene (Gene ID: 1869) in human. E2F-1 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5′-TTTC[CG]CGC-3′ found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. It is a component of the DRTF1/E2F transcription factor complex and forms heterodimers with DP family members. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1 complex is shown to bind specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase and detaches from the DRTF1/E2F complex that renders E2F transcriptionally active. E2F-1 can be phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It is also phosphorylated at serine 364 by CHEK2, which stabilizes it upon DNA damage and regulates its effect on transcription and apoptosis. Acetylation of E2F-1 by P/CAF/KAT2B stimulates its DNA-binding.

特異性

Clone 71-10 is a rabbit monoclonal antibody that specifically detects human E2F1 phosphorylated on Serine 375.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids surrounding phosphoserine 375 from the C-terminal region of human transcription factor E2F1.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Anti-phospho-E2F1 (Ser375), clone 71-10, Cat. No. MABE1782, is a highly specific rabbit monoclonal antibody that targets Transcription factor E2F1 phosphorylated on Serine 375 and has been tested in ELISA and Western Blotting.

ELISA Analysis: A representative lot detected phospho-E2F1 (Ser375) in ELISA applications (Dale, T., et. al. (2015). Nat Chem Biol. 11(12):973-980).

品質

Evaluated by Western Blotting in HEK293 cells transfected with pcDNA3.1 empty vector, HEK 293 cells transfected with E2F1 wild type-GST, HEK 293 cells transfected with E2F1 wild type-GST with alanine substituted for serine at 375.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected phospho-E2F1 (Ser375) in HEK293 cells transfected with E2F1 wild type-GST,, but not in HEK 293 cells transfected with E2F1 wild type-GST with alanine substituted for serine at 375.

ターゲットの説明

~80 kDa observed ( GST fusion protein); 46.92 kDa calculated (without GST). Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified rabbit monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.