MAB1285F

Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated

clone HLA.ABC.m3, Chemicon^®, from mouse

• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: FITC conjugate
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: HLA.ABC.m3, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Chemicon^®
• テクニック: flow cytometry: suitable; immunofluorescence: suitable
• アイソタイプ: IgG2a
• NCBIアクセッション番号: NM_005514.5
• UniProtアクセッション番号: P01889
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HLA-B(3106)

詳細

詳細

BIOCHEMISTRY: pI = 8.0

The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.

特異性

This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7

(9.5x107M-1).

免疫原

Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A.

アプリケーション

Research Category
炎症及び免疫

Research Sub Category
免疫グロブリン及び免疫

Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated detects level of HLA-B27 & has been published & validated for use in FC & IF.

This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.

SUGGESTED USAGE DILUTION

Flow cytometry and immunofluorescence 1:15 to 1:60

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

物理的形状

Purified from mouse ascitic fluid. Fluorescein conjugated. The antibody is supplied in 0.5ml phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.

保管および安定性

Store at 2 to 8°C. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable