HTS134M

ChemiSCREEN Membrane Preparation Recombinant Human GIP Glucagon Family Receptor

• UNSPSCコード: 41123201
• eCl@ss: 32161000

特性

• 詳細: GPCR Class B; protein target GIP
• 品質水準: 100
• テクニック: radioligand binding assay (RLBA): suitable
• UniProtアクセッション番号: P48546
• 遺伝子情報: human ... GIPR(2696)

詳細

詳細

FUNCTION: SwissProt: P48546 # This is a receptor for GIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 466 amino acids; 53157 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P48546 ## Belongs to the G-protein coupled receptor 2 family.

アプリケーション

Radioligand binding assay

物理的形状

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservRC:R[590]Catives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

保管および安定性

Store at –80°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.

アナリシスノート

Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl₂, 1 mM CaCl₂, 0.2% BSA, filtered and stored at 4°C
Radioligand: [¹²⁵I] GIP (Perkin Elmer#: NEX-402)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 8-fold signal:background with ¹²⁵I-labeled GIP at 0.2 nM

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2