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由来生物
human
品質水準
リコンビナント
expressed in Chem-1 cells
包装
pkg of 1 mL
メーカー/製品名
ChemiScreen
Chemicon®
濃度
1 mg/mL
テクニック
ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
詳細
CX3CR1 is a GPCR expressed on natural killer cells, cytotoxic T lymphocytes, and macrophages. The sole ligand for CX3CR1, fractalkine, is an unusual chemokine that is expressed as a transmembrane molecule with a CX3C domain and a mucin domain (Imai et al., 1997). Fractalkine is highly expressed on endothelial cells activated by TNFa and other proinflammatory cytokines, and fractalkine/CX3CR1 interactions mediate recruitment of macrophages into the atherosclerotic plaque (Lesnick et al., 2003; McDermott et al., 2003). In addition, fractalkine and CX3CR1 have been implicated in the pathogenesis of glomerulonephritis, HIV infection and rheumatoid arthritis (Ito et al., 2002; Faure et al., 2003; Nanki et al., 2002). Chemicon′s CX3CR1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of fractalkine/CX3CR1 interactions. The membrane preparations exhibit a Kd of 0.05 nM for [125I]-fractalkine. With 2.5 mg/well CX3CR1 Membrane Prep and 0.05 nM [125I]-fractalkine, a greater than 7-fold signal-to-background ratio is obtained.
Full-length Human CX3CR1
アプリケーション
Human CX3CR1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Radioligand binding assay, and GTPγS binding.
生物化学的/生理学的作用
GPCR Class: A
Protein Target: CX3CR1
Target Sub-Family: Chemokine
品質
Bmax: 3.13 pmol/mg
Kd: 0.05 nM
Kd: 0.05 nM
規格
Inucbation Conditions
RECOMMENDED ASSAY CONDITIONS:
Binding buffer: 50mM HEPES, 5mM MgCl2, 1mM CaCl2, 0.2% BSA, pH to 7.4 and filter.
Radioligand: [125I] Fractalkine (PerkinElmer#: NEX368)
One vial contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 5-fold signal:background with 125I-labeled fractalkine at 0.05 nM.
RECOMMENDED ASSAY CONDITIONS:
Binding buffer: 50mM HEPES, 5mM MgCl2, 1mM CaCl2, 0.2% BSA, pH to 7.4 and filter.
Radioligand: [125I] Fractalkine (PerkinElmer#: NEX368)
One vial contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 5-fold signal:background with 125I-labeled fractalkine at 0.05 nM.
物理的形状
Packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA
Packaging method: Membranes protein were adjusted to 1 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C.
Packaging method: Membranes protein were adjusted to 1 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C.
保管および安定性
Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
HTS015M:
試験成績書(COA)
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