GR15

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

liquid, clone EGFR.1, Calbiochem^®

• 別名: Anti-Epidermal Growth Factor Receptor
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: EGFR.1, monoclonal
• 形状: liquid
• 含みます: ≤0.1% sodium azide as preservative
• 化学種の反応性: human
• 以下との反応禁止: rat, mouse
• メーカー／製品名: Calbiochem^®
• 保管条件: do not freeze
• アイソタイプ: IgG2b
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EGFR(1956) ; mouse ... Egfr(13649) ; rat ... Egf(25313)

詳細

詳細

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.

Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended.

免疫原

Human

partially purified, human EGFR from A431 cells

アプリケーション

Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; use Cat. No. GR15L; see application references)

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

物理的形状

In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.

アナリシスノート

Negative Control
HL-60 cells

Positive Control
A-431 cells

その他情報

EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.

Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable