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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
UCHT1, monoclonal
交差性
human
テクニック
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CD3E(916)
詳細
T-cell surface glycoprotein CD3 epsilon chain (UniProt P07766; also known as CD3-epsilon, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain) is encoded by the CD3E (also known as T3E) gene (Gene ID 916) in human. The T cell receptor or TCR is a plasma membrane protein complex responsible for recognizing antigens bound to major histocompatibility complex (MHC) on antigen presenting cells. In 95% of T cells, the TCR is composed of a disulfide-linked alpha (α) and beta (β) dimer, while among 5% of T cells the TCR is composed of a gamma (γ) and a delta (δ) dimer. T cells are activated when their surface TCRs engage with antigen/MHC complex on antigen presenting cells. Each TCR alpha and beta chain contains a variable (V) and a constant (C) extracellular immunoglobulin-like domain, followed by a transmembrane region and a short cytoplasmic tail. The alpha and beta chains have very short cytoplasmic tail and rely on the CD3 (cluster of differentiation 3) co-receptor complex for transducing signaling further intracellularly. The CD3 co-receptor complex is composed of a CD3γ chain, a CD3δ chain, and two CD3ε chains. The CD3 complex, TCR dimer, and a ζ-chain together comprise the functional TCR complex. The CD3 epsilon chain is initially produced with a signal peptide (a.a. 1-22), the removal of which yields the mature chain (a.a. 23-207) with an Ig-like (a.a. 32-112) domain-containing extracellular region (a.a. 23-126), a transmembrane segment (a.a. 127-152), and a cytoplasmic tail (a.a. 153-207), which contains the immunoreceptor tyrosine-based activation motif or ITAM (a.a. 178-205) that is essential for mediating ZAP-70 interaction and transducing TCR signaling.
特異性
Clone UCHT1 reacts with the extracellular domain of human CD3 epsilon chain (Clevers, H., et al. (1988). Eur. J. Immunol. 18(5):705-710).
免疫原
Epitope: Extracellular domain.
Human infant thymocytes and lymphocytes from a patient with Sézary disease.
アプリケーション
Research Category
炎症及び免疫
炎症及び免疫
Research Sub Category
免疫シグナル伝達
免疫シグナル伝達
Anti-CD3e Antibody, clone UCHT1 is an antibody against CD3e for use in Flow Cytometry, Immunoprecipitation, ELISA, Immunocytochemistry, Immunohistochemistry, Affects Function.
Immunoprecipitation Analysis: Representative lots immunoprecipitated CD3 epsilon-associated TCR complex (Schamel, W.W., et al. (2005). J. Exp. Med. 202(4):493-503; Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953).
ELISA Analysis: A representative lot was used as the capture antibody for the detection of epsilon-associated TCR complex proteins by sandwich ELISA (Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953).
Immunocytochemistry Analysis: A representative lot detected the exogensously expressed human CD3 epsilon chain on the surface of murine T cells by flourescent immunocytochemistry (Clevers, H., et al. (1988). Eur. J. Immunol. 18(5):705-710).
Immunocytochemistry Analysis: A representative lot was employed to determined the plasma membrane and cytoplasmic CD3 immunoreactivity in T cell populations and T-ALL blast cells by flourescent immunocytochemistry (Campana, D., et al. (1987). J. Immunol. 138(2):648-655).
Immunohistochemistry Analysis: A representative lot detected CD3+ T cells (T3+) in the periglandular stromal aggregates in frozen dometrium sections from female donors undergone routine dilation and curettage procedures (Kamat, B.R., and Isaacson, P.G. (1987). Am. J. Pathol. 127(1):66-73).
Affects Function: A representative lot induced surface IL-2R expression and proliferation of human peripheral blood T lymphocytes (Van Wauwe, J.P., et al. (1984). J. Immunol. 133(1):129-132).
Flow Cytometry Analysis: A representative lot was employed for fluorescence activated cell sorting (FACS) of CD3+ human lymphocytes (Beverley, P.C., and Callard, R.E. (1981). Science. 11(4):329-334).
ELISA Analysis: A representative lot was used as the capture antibody for the detection of epsilon-associated TCR complex proteins by sandwich ELISA (Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953).
Immunocytochemistry Analysis: A representative lot detected the exogensously expressed human CD3 epsilon chain on the surface of murine T cells by flourescent immunocytochemistry (Clevers, H., et al. (1988). Eur. J. Immunol. 18(5):705-710).
Immunocytochemistry Analysis: A representative lot was employed to determined the plasma membrane and cytoplasmic CD3 immunoreactivity in T cell populations and T-ALL blast cells by flourescent immunocytochemistry (Campana, D., et al. (1987). J. Immunol. 138(2):648-655).
Immunohistochemistry Analysis: A representative lot detected CD3+ T cells (T3+) in the periglandular stromal aggregates in frozen dometrium sections from female donors undergone routine dilation and curettage procedures (Kamat, B.R., and Isaacson, P.G. (1987). Am. J. Pathol. 127(1):66-73).
Affects Function: A representative lot induced surface IL-2R expression and proliferation of human peripheral blood T lymphocytes (Van Wauwe, J.P., et al. (1984). J. Immunol. 133(1):129-132).
Flow Cytometry Analysis: A representative lot was employed for fluorescence activated cell sorting (FACS) of CD3+ human lymphocytes (Beverley, P.C., and Callard, R.E. (1981). Science. 11(4):329-334).
品質
Evaluated by Flow Cytometry in human PBMC.
Flow Cytometry Analysis: 0.1 µg of this antibody detected CD3e in 1X10E6 human PBMC.
Flow Cytometry Analysis: 0.1 µg of this antibody detected CD3e in 1X10E6 human PBMC.
ターゲットの説明
23.15 kDa (Proform) and 20.74 (mature form) calculated. ~27 kDa reported in UCHT1 immunoprecipitate (Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953).
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in PBS without preservatives.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
CBL150-I:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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