AQ303F

Goat Anti-Mouse IgG Antibody, F(ab′)2, FITC conjugate

1 mg/mL, Chemicon^®

• 別名: Goat anti-Mouse IgG FITC
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

特性

• 由来生物: goat
• 品質水準: 100
• 結合体: FITC conjugate
• 抗体製品の状態: F(ab′)₂ fragment of affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• 交差性: mouse
• メーカー／製品名: Chemicon^®
• 濃度: 1 mg/mL
• テクニック: immunofluorescence: suitable
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

The reagent is an anti-mouse antibody F(ab′)2 fragment from goat. The F(ab′)2 fragments are obtained by pepsin cleavage. The fragments are conjugated to fluorescein isothiocyanate and stabilized in buffer. The molar ratio of fluorescein: protein is: 3 - 7.

PURIFICATION: The goat-lgG was purified by affinity chromatography and absorbed to remove cross-reactivity to human immunoglobulins.

特異性

Specific for mouse IgG, heavy and light chain. The cross-reactivities of the anti-mouse IgG antibody were tested in an ELISA. Minimum cross-reactivity to human IgG.

アプリケーション

Research Category
二次抗体及びコントロール抗体

Research Sub Category
フラグメント特異的二次抗体

Immunohistochemistry: 1:200-1:500 (Coligan et al. 1997; Harlow & Lane 1988; Bullock & Petrusz 1982; Javois 1994)

Immunocytochemistry: 1:200-1:500 (Coligan et al. 1997; Harlow & Lane 1988; Bullock & Petrusz 1982; Javois 1994)

Flow cytometery: 1 μg per 1 x 10E6 cells (Coligan et al. 1997; Harlow & Lane 1988; Javois 1994) FITC absorption peak is 488-492nm, its emission peak is 520nm.

Optimal working dilutions must be determined by the end user.

This Goat anti-Mouse IgG Antibody, F(ab′)2, FITC conjugate is validated for use in IF for the detection of Mouse IgG.

物理的形状

ImmunoAffinity Purified

Liquid in 0.02M Phosphate Buffer. 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA and 0.01% Sodium azide.

保管および安定性

The antibody conjugate solution is stable at 2–8°C for 12 months. Do not store in a diluted format.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable