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メーカー／製品名
BLACK-GOLD
テクニック
immunohistochemistry: suitable
輸送温度
ambient
詳細
Black-Gold II is an aurohalophosphate complex which stains specifically for myelin within the central nervous system. Black-Gold II staining provides high resolution, high contrast, a short histochemical processing time, and high reproducibility. Black-Gold II is a new and improved version of its predecessor, Black-Gold (Cat # AG390). The advantages of Millipore’s Black-Gold II Myelin Staining Kit over Black Gold I are that it is more readily soluble, can be utilized at a higher concentration, produces a more uniform and consistent staining, takes less time to stain, and does not require post-staining intensification procedures.
The use of Black-Gold II is tailored to studies using formalin-fixed, non-solvent processed tissue. The technique stains large myelinated tracts dark red-brown, while the individual myelinated fibers appear black. This novel tracer can be used to localize both normal and pathological myelin. Black-Gold II can demonstrate and characterize specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, methamphetamine, acrylamide, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid.
The Black-Gold II Myelin Staining Kit also comes with sodium thiosulfate and cresyl violet. The sodium thiosulfate solution is required for extracting excess Black-Gold II stain. Whereas the cresyl violet is a Nissl stain that colors cell bodies a brilliant bluish hue and is used as a counter stain with Black-Gold II.
Black Gold II Purity: No detectable amount of uncomplexed gold was found.
Black Gold II Illumination: Bright field or dark field.
Black Gold II Solubility: Freely soluble in water, saline, or dilute acids.
The use of Black-Gold II is tailored to studies using formalin-fixed, non-solvent processed tissue. The technique stains large myelinated tracts dark red-brown, while the individual myelinated fibers appear black. This novel tracer can be used to localize both normal and pathological myelin. Black-Gold II can demonstrate and characterize specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, methamphetamine, acrylamide, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid.
The Black-Gold II Myelin Staining Kit also comes with sodium thiosulfate and cresyl violet. The sodium thiosulfate solution is required for extracting excess Black-Gold II stain. Whereas the cresyl violet is a Nissl stain that colors cell bodies a brilliant bluish hue and is used as a counter stain with Black-Gold II.
Black Gold II Purity: No detectable amount of uncomplexed gold was found.
Black Gold II Illumination: Bright field or dark field.
Black Gold II Solubility: Freely soluble in water, saline, or dilute acids.
構成
Black-Gold II: 150mg dry powder, yellow in color.
Cresyl Violet Stain: 150mg dry powder, purplish-black in color.
5% Sodium Thiosulfate: 10ml
Cresyl Violet Stain: 150mg dry powder, purplish-black in color.
5% Sodium Thiosulfate: 10ml
物理的形状
See Components
保管および安定性
The kit can be stored at room temperature. Ideally, the Black-Gold II powder should be kept in a desiccator, because of its hygroscopic nature. The dry powder, if unopened and stored properly, is useable for up to one year from date of receipt. Once resuspended the Black-Gold II staining solution should be stored in the dark at 4°C and is good for up to 3 months.
TOXICITY: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not intended for human consumption.
TOXICITY: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not intended for human consumption.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AG105:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Farshid Sepehrband et al.
NMR in biomedicine, 29(3), 293-308 (2016-01-11)
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Introducing Black-Gold II, a highly soluble gold phosphate complex with several unique advantages for the histochemical localization of myelin.
Schmued, Larry, et al.
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L Schmued et al.
Brain research, 837(1-2), 289-297 (1999-08-06)
A novel haloaurophosphate complex called Black-Gold has been synthesized and applied to localize myelin within the central nervous system. The technique is tailored to studies using formalin fixed non-solvent processed tissue. The technique stains large myelinated tracts dark red-brown, while
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The cuprizone model is a well-established and investigated paradigm to study demyelination and remyelination in rodents. Cuprizone is usually administrated by mixing in the powdered or pelleted rodent chow. However, since cuprizone is sensitive to the environment and the consumption
Neurotoxic-related changes in tyrosine hydroxylase, microglia, myelin, and the blood-brain barrier in the caudate-putamen from acute methamphetamine exposure.
Bowyer, John F, et al.
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