ABT58

Anti-phospho PKA-RII (Ser96) Antibody

from rabbit, purified by affinity chromatography

• 別名: PRKAR2A, protein kinase, cAMP-dependent, regulatory, type II, alpha
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human, rat
• 化学種の反応性（ホモロジーによる予測）: mouse (based on 100% sequence homology)
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_032950
• UniProtアクセッション番号: P12367
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer96)
• 遺伝子情報: human ... PRKAR2A(5576)

詳細

詳細

cAMP-dependent protein kinase type II-alpha regulatory subunit (PKA-RIIα) is an enzymatic protein belonging to the cAMP-dependent kinase regulatory chain family, a group of regulatory proteins involved in the PKA signaling pathway. This pathway has been shown to have regulatory roles in development, growth, and mortality of various organisms. PKA-RIIα is a subunit of the PKA type II regulatory chain, one of two PKA isoforms, and is involved in the regulation of membrane interactions through its binding to anchoring proteins, such as MAP2 kinase. Before its activation with cAMP, the enzyme is comprised of two catalytic chains and two regulatory chains, which split into two catalytic monomers and one regulatory dimmer after interacting with cAMP.

特異性

The antibody recognizes PKA-RIIalpha when phosphorylated at Ser96.

免疫原

Epitope: Phosphorylated Ser96 of PKA-RIIalpha

KLH-conjugated linear peptide corresponding to mouse PKA-RIIalpha phosphorylated at Ser96.

アプリケーション

Research Category
細胞骨格

細胞シグナル伝達

Research Sub Category
細胞骨格シグナル伝達

キナーゼ及びホスファターゼ

This Anti-phospho PKA-RII (Ser96) Antibody is validated for use in WB for the detection of phospho PKA-RII (Ser96).

品質

Evaluated by Western Blot in Forskolin untreated and treated PC12 cell lysates.

Western Blot Analysis: 1 µg/mL of the antibody detected PKA-RIIalpha (Ser96) in 10 µg of Forskolin untreated and treated PC12 cell lysates.

ターゲットの説明

~ 51 kDa MW observed. The specificity of the target was shown by Lambda phosphatase treatment.

関連事項

Replaces: 06-704

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Forskolin untreated and treated PC12 cell lysates

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable