ABT135

Anti-phospho-FAK (Tyr397) Antibody

from rabbit, purified by affinity chromatography

• 別名: Focal adhesion kinase 1, FADK 1, Protein-tyrosine kinase 2, pp125FAK
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: rat
• 化学種の反応性（ホモロジーによる予測）: human (based on 100% sequence homology), mouse (based on 100% sequence homology), Xenopus (based on 100% sequence homology), chicken (based on 100% sequence homology)
• テクニック: cell based assay: suitable; western blot: suitable
• NCBIアクセッション番号: NP_722560
• UniProtアクセッション番号: Q05397
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pTyr397)
• 遺伝子情報: human ... PTK2(5747)

詳細

詳細

Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397.

特異性

This antibody recognizes FAK phosphorylated at Tyr397.

免疫原

Epitope: Phosphorylated Tyr397

KLH-conjugated linear peptide corresponding to human FAK phosphorylated at Tyr397.

アプリケーション

Research Category
細胞骨格

Research Sub Category
接着分子（CAM）

Detect phospho-FAK (Tyr397) using this Anti-phospho-FAK (Tyr397) Antibody validated for use in WB, Cell Function Assay.

Peptide Inhibition Assay Analysis: A 1:1,000 dilution from a representative lot peptide blocked on RAT2 cells transfected with Src.

品質

Evaluated by Western Blot in untransfected and Src-transfected RAT2 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-FAK (Tyr397) in 10 µg of untransfected and Src-transfected RAT2 cell lysates.

ターゲットの説明

~125 kDa observed.
An uncharacterized band appears at ~190 kDa in some lysates.

関連事項

Replaces: 04-974

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
untransfected and Src-transfected RAT2 cell lysates

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable