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Key Documents

ABS1848

Sigma-Aldrich

Anti-Rho (-A, -B, -C) Antibody

from rabbit, purified by affinity chromatography

別名:

Transforming protein RhoA, h12, Rho-related GTP-binding protein RhoB, h6, Rho-related GTP-binding protein RhoC, h9

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702

由来生物

rabbit

品質水準

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

primary antibodies

クローン

polyclonal

精製方法

affinity chromatography

化学種の反応性

human

化学種の反応性(ホモロジーによる予測)

chicken (based on 100% sequence homology), rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), mouse (based on 100% sequence homology), nematode (based on 100% sequence homology), Xenopus (based on 100% sequence homology)

テクニック

western blot: suitable

NCBIアクセッション番号

UniProtアクセッション番号

ターゲットの翻訳後修飾

unmodified

遺伝子情報

詳細

Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2).

特異性

This polyclonal antibody is raised against an internal sequence highly conserved among RhoA (100%), RhoB (95%), and RhoC (100%).

免疫原

Epitope: Amino acids 73-94.
KLH-conjugated synthetic peptide (SYPDTDVILMCFSIDSPDSLEN-KK) corresponding to amino acids 73-94 of human RhoA & RhoB, with two C-terminal lysines added to increase peptide solubility corresponding to human Rho (-A, -B, -C).

アプリケーション

Research Category
細胞シグナル伝達
Research Sub Category
Gタンパク質
Anti-Rho (-A, -B, -C) Antibody is an antibody against Rho (-A, -B, -C) for use in Western Blotting.

品質

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected RhoA family GTPase in 10 µg of HL-60 cell lysate.

ターゲットの説明

~22 kDa observed. 21.44 kDa (human RhoA), 21.78 kDa (human RhoB), and 21.68 kDa (human RhoC) calculated.

物理的形状

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


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