ABN1681

Anti-Phospho-PANX1 (Tyr198)

from rabbit

• 別名: Pannexin-1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• テクニック: immunofluorescence: suitable; western blot: suitable
• NCBIアクセッション番号: NP_062355
• UniProtアクセッション番号: Q9JIP4
• 輸送温度: ambient
• ターゲットの翻訳後修飾: phosphorylation (pTyr198)
• 遺伝子情報: human ... PANX1(24145)

詳細

詳細

Pannexin-1 (UniProt: Q9JIP4) is encoded by the Panx1 gene (Gene ID: 55991) in murine species. Pannexin-1 is a multi-pass membrane protein of the pannexin family that serves as a high-conductance, voltage-gated channel. It is widely expressed, including in cartilage, skin, spleen, heart, and brain. In the nervous system Panx1 transcripts have been detected in the cerebellum, cortex, lens, retina, pyramidal cells, interneurons of the neocortex and hippocampus. Three pannexin family genes (PANX1-3) have been identified in murine and human genomes. Pannexins share structural and functional similarities and play important roles in several physiological processes, including ATP release, Ca2+ homeostasis, and synaptic neurotransmitters release. Pannexins can also form intermixed channels and the intermixing is more evident between Pannexin 1 and 2. The level of intermixing is governed by the degree of N-glycosylation. Post-translational N-glycosylation plays a role in cell surface targeting. Pannexin-1 can also undergo S-nitrosylation, which can inhibit channel currents and ATP release. It has been shown that Pannexin-1 channel can release ATP in response to activation by TNF-alpha that requires activation of type-1 TNF receptors and downstream signaling through Src family kinases (SFK). Tryosine 198 in the intracellular loop region of Pannexin-1 is highly conserved and is considered as a SFK phosphorylation site. Pannexin-1 has been linked to propagation of calcium waves, regulation of vascular tone, taste-bud function, and in tumor suppression in gliomas. Pannexin-1 channel opening can contribute to cell death and seizures under ischemic or epileptic conditions. (Ref.: Penuela, S et al. (2013). Biochim. Biophys. Acta 1828(11); 15-22).

特異性

This polyclonal antibody specifically detects pannexin-1 phosphorylated on tyrosine 198 in HEK293 cells transfected with murine Pannexin 1.

免疫原

A linear peptide corresponding to 12 amino acids surrounding phospho-Tyr198 of murine Pannexin-1.

Epitope: cytoplasmic domain

アプリケーション

Research Category
ニューロサイエンス

Anti-Phospho-PANX1 (Tyr198), Cat. No. ABN1681, is a highly specific rabbit polyclonal antibody that targets Pannexin 1 and has been tested in Immunofluorescence and Western blotting.

Western Blotting Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in human coronary smooth muscle cells transfected with an epitope tagged Panx1, & recombinant human Pannexin1 and (active) human Src tyrosine kinase. (Courtesy of Isakson Lab, University of Virginia, Charlottesville, VA).

Immunofluorescence Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in isolated mesenteric venule cross-sections from mice (Lohman, A.W., et. al. (2015). Nat. Commun. 6:7965).

Western Blotting Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in HUVECs stimulated with TNF-a (Lohman, A.W., et. al. (2015). Nat Commun. 6:7965).

品質

Evaluated by Western Blotting in transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Phospho-PANX1 (Tyr198) in 10 µg of lysate from HEK293 cells transfected with murine pannexin-1.

ターゲットの説明

~58 kDa observed; 48.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 8.0), 0.1% BSA without azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable