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由来生物
rabbit
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
交差性
human
包装
antibody small pack of 25 μL
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG
ターゲットの翻訳後修飾
unmodified
詳細
Huntington disease (HD) is a progressive neurodegenerative disorder is caused by a CAG,CTG expansion in the HTT gene that results in the production of a mutant huntingtin protein (HTT) with polymeric expansions that accumulate in human brain with HD. PolyCys is an antisense repeat-associated non-ATG (RAN) translation protein that accumulate in various brain regions with toxic effects leading to microglial activation and neuronal loss. HD-RAN polyCys toxicity is shown to be comparable to that of polyGln. HD-RAN proteins are abundant in regions of the brain that are most affected by HD and these regions display pathologic features of HD, such as caspase-3 activity and microglial activation. HD-Ran protein accumulation and aggregation in HD brains are shown to be length dependent.
特異性
This rabbit polyclonal antibody specifically detects RAN proteins with PolyCys.
免疫原
A synthetic peptide corresponding to the C-terminus of the predicted polyCys frame of HD in the CTG direction (alpha-HD-Cys-Ct).
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Anti-HD-polyCys-Ct, Cat. No. ABN1671, is a highly specific rabbit polyclonal antibody that targets HD-polyCys-Ct and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected HD-polyCys-Ct in Immunocytochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
Western Blotting Analysis: A representative lot detected HD-polyCys-Ct in Western Blotting applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
Western Blotting Analysis: A 1:750 dilution form a representative lot detected HD-polyCys-Ct in HEK293T cells transfected with HD-polyCys-Ct.
Immunohistochemistry Analysis: A representative lot detected HD-polyCys-Ct in Immunohistochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
Western Blotting Analysis: A representative lot detected HD-polyCys-Ct in Western Blotting applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
Western Blotting Analysis: A 1:750 dilution form a representative lot detected HD-polyCys-Ct in HEK293T cells transfected with HD-polyCys-Ct.
Immunohistochemistry Analysis: A representative lot detected HD-polyCys-Ct in Immunohistochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).
品質
Evaluated by Western Blotting in HEK293Tcells transfected with HD-PolyCys-Ct.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected in PolyCys in HEK293T cells transfected with HD-polyCys-Ct, but not in control cells.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected in PolyCys in HEK293T cells transfected with HD-polyCys-Ct, but not in control cells.
ターゲットの説明
~50 kda observed. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Monica Bañez-Coronel et al.
Neuron, 88(4), 667-677 (2015-11-22)
Huntington disease (HD) is caused by a CAG ⋅ CTG expansion in the huntingtin (HTT) gene. While most research has focused on the HTT polyGln-expansion protein, we demonstrate that four additional, novel, homopolymeric expansion proteins (polyAla, polySer, polyLeu, and polyCys)
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