AB5731

Anti-Nanog Antibody, NT

Chemicon^®, from rabbit

• 別名: Homeobox transcription factor Nanog, Nanog homeobox
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human, rat, mouse
• 包装: antibody small pack of 25 μg
• メーカー／製品名: Chemicon^®
• テクニック: western blot: suitable
• 入力: sample type: mouse embryonic stem cell(s); sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• アイソタイプ: IgG
• NCBIアクセッション番号: NM_024865.2
• UniProtアクセッション番号: Q9H9S0
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NANOG(79923) ; mouse ... Nanog(71950)

詳細

詳細

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).

特異性

Antibody recognizes Nanog.

免疫原

Epitope: N-terminus

Synthetic peptide from mouse Nanog.

アプリケーション

Research Category
幹細胞研究

Research Sub Category
多能性及び初期分化

Detect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB.

Western blotting:
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.

品質

Routinely evaluated by Western Blot on Embryonic Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 μg of Embryonic Mouse Brain lysates

ターゲットの説明

39 kDa (MW 34 kDa)

物理的形状

Affinity purified

Purified rabbit polyclonal immunoglobulin in buffer containing liquid 0.1% sodium azide.

保管および安定性

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

アナリシスノート

Control
Whole cell lysate from mouse embryonic stem cells.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.