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由来生物
rabbit
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
mouse, rat, human
包装
antibody small pack of 25 μg
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
UniProtアクセッション番号
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SLC17A8(246213)
詳細
Vesicular glutamate transporter 3 (UniProt Q7TSF2; also known as Solute carrier family 17 member 8, VGluT3) is encoded by the Slc17a8 (also known as Vglut3) gene (Gene ID 266767) in rat species. VGluTs are proton-dependent carriers that are responsible for the upload of the major excitatory neurotransmitter glutamate into synaptic vesicles within presynaptic terminals to allow later regulated release at the synaptic cleft. VGluT3 is a multi-pass synaptic vesicle membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neurons. Rat VGluT3 has 12 helical, 7 cytoplasmic, and 6 vesicular domains. It is structurally similar to VGluT1 and -2, but differs in its distribution pattern. Unlike VGluT1 and 2 that rely on interactions with the endocytic machinery for recycling, but remain almost exclusively axonal, VGluT3 can target to the cell body and dendrites, as well as the axon of certain neurons and is implicated in the retrograde synaptic release of glutamate. VGluT3 displays a very restricted localization and is not present in known glutamatergic neurons, but is found in cholinergic neurons in the caudate-putamen and in serotonergic neurons in the raphe nucleus. VGluT3 is localized over vesicle clusters in both symmetric (inhibitory or modulatory) and asymmetric (excitatory) synapses. (Ref.: Herzog, E et al. (2004). Neuroscience. 123(4):983-1002; Seal, RP et al. (2008). Neuron 57(2); 263-275).
特異性
This rabbit polyclonal antibody specifically detects Vesicular glutamate transporter 3(VGluT3). It targets an epitope with in 13 amino acids from the C-terminal region.
免疫原
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of rat Vesicular glutamate transporter 3 (VGluT3).
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Anti-Vesicular Glutamate Transporter 3 (VGLUT3), Cat. No. AB5421-II, is a highly specific rabbit polyclonal antibody that targets Vesicular glutamate transporter 3 and has been tested foe use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in rat brain and human brain tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in rat brain and human brain tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cells.
品質
Evaluated by Western Blotting in PC12 tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Vesicular Glutamate Transporter 3 (VGLUT3) in PC12 cell lysate.
ターゲットの説明
~ 60 kDa observed; 64.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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