AB4332

Anti-acetyl-MVH (Lys405) Antibody

from rabbit, purified by affinity chromatography

• 別名: Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Mvh, Vasa homolog
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: pig, human, mouse
• 化学種の反応性（ホモロジーによる予測）: porcine (based on 100% sequence homology), rat (based on 100% sequence homology)
• テクニック: dot blot: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable (peptide); western blot: suitable
• NCBIアクセッション番号: NP_034159
• UniProtアクセッション番号: Q61496
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: acetylation (Lys405)
• 遺伝子情報: mouse ... Ddx4(13206)

詳細

詳細

MVH (also known as DDX4 or Vasa) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. MVH is mostly localized in testicular germ cells in the cytoplasm of spermatocytes. MVH is a high-copy extragenic suppressor gene. MVH is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility and in stem cell development.

特異性

This antibody specifically recognizes wild type MVH acetylated at Lys405, but not the Lys405 mutant.

免疫原

Epitope: Acetylated Lys405

KLH-conjugated linear peptide corresponding to mouse MVH acetylated at Lys405.

アプリケーション

Research Category
幹細胞研究

エピジェネティクス及び核内機能分子

Research Sub Category
細胞周期、DNA複製及び修復

Anti-acetyl-MVH (Lys405) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-MVH (Lys405) also known as Probable ATP-dependent RNA helicase DDX4, DEAD box protein & has been validated in DB, WB, ICC, PIA & IP.

Western Blot/Immunoprecipitation Analysis: A representative lot detected Flag-tagged acetyl-MVH (Lys405) in the presence of Myc-HAT/p46.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected acetyl-MVH (Lys405) in mouse germ cells (I. Nagamori, Department of Pharmacology, University of California, Irvine).

品質

Evaluated by Dot Blot/Peptide Inhibition Assay in non-acetylated and acetylated MVH (Lys405).

Dot Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected acetyl-MVH (Lys405) peptide.

ターゲットの説明

76 kDa calculated

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Non-acetylated and acetylated MVH (Lys405)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids