AB3403-I

Anti-Beta Galactosidase Antibody, E. coli

from chicken, purified by affinity chromatography

• 別名: Beta-galactosidase, Acid beta-galactosidase, Lactase, Elastin receptor 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: chicken
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: bacteria
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_752394
• UniProtアクセッション番号: P16278
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: Escherichia coli K12 ... Lacz(945006)

詳細

詳細

β Galactosidase, also known as Beta-galactosidase, Beta-gal, LacZ, or Lactase, and encoded by the gene lacZ, is the enzyme in bacteria that hydrolyzes galactosides (sugars chains containing galactose) in to monosaccharides. β Galactosidase is typically an extracellularly produced enzyme that hydrolyzes the beta-glycosidic bond between galactose and another organic moiety. In E. coli, the β Galactosidase gene is present as part of the inducible lac operon system which is activated in the presence of lactose when glucose level is low. Today, bacterial beta galactosidase is used in molecular biology as a reporter marker to monitor gene expression, and because the enzyme has two functional domains, it forms the basis for α-complementation which is the methodology behind for the blue/white screening of recombinant clones. The presence or absence of an active β-galactosidase may be detected by X-gal, which produces a characteristic blue dye when cleaved by β-galactosidase, thereby providing an easy means of distinguishing the presence or absence of cloned product in a plasmid.

免疫原

Recombinant protein corresponding to E. Coli Beta Galactosidase.

アプリケーション

Anti-Beta Galactosidase Antibody, E. coli is a highly specific chicken polyclonal antibody & that targets β-Galactosidase & has been tested in western blotting.

品質

Evaluated by Western Blotting in TOP10/PCU19 E. Coli lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Beta Galactosidase in 10 µg TOP10/PCU19 E. Coli lysate.

ターゲットの説明

~120 kDa observed

関連事項

Replaces: AB3403

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable