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由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, mouse, rat
メーカー／製品名
Chemicon®
テクニック
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ITGB4(3691)
mouse ... Itgb4(192897)
rat ... Itgb4(25724)
特異性
Specific for beta4 integrin as determined by immunoprecipitation. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. No cross-reactivity to other integrin b subunits.
免疫原
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human β4 integrin subunit (Tamura et al. 1990). The sequence used is NH2-(K)GTLSTHMDQQFFQT-amide. The lysine (K) is not in the β4 sequence and was added to conjugate the peptid
アプリケーション
Anti-Integrin β4 Antibody is an antibody against Integrin β4 for use in IP, IH.
Immunoprecipitation from labeled cell extracts: 5 μL of serum is sufficient to precipitate alpha6beta4 from 1-2 x 10E6 cells. In some cases immunoprecipitation from 35S methionine labeled cells results in two bands of 95 and 55 kDa of unknown origin in addition to the 200 kDa and 140 kDa bands corresponding to beta4 and alpha6.
Immunohistochemistry: 5 μg/mL. See protocol below.
Optimal working dilutions must be determined by the end user.
Suggested Protocol for Immunohistochemical:
1. Fix cryostat sections with a 1:1 chloroform-acetone mixture for 10 minutes at room temperature.
2. Air Dry.
3. Wash with 2 mL of 1x PBS.
4. Add 200 μL of first antibody (5 μg/mL) to each slide. Incubate for 1 hour at room temperature in a humidity chamber.
5. Wash with 5 mL of 1x PBS.
6. Add appropriately diluted anti-rabbit secondary antibody conjugate and incubate for 1 hour in a humidity chamber
7. Wash with 5 mL of 1x PBS.
8. Add 200 μL of development solution (containing DAB or other substrate depending on the secondary antibody) and incubate at room temperature for the necessary time to develop the dark color. Follow the reaction with a microscope, to establish the correct color intensity.
9. Block the reaction by washing with 2 mL of 1x PBS.
10. Counterstain with hematoxylin as desired.
Immunohistochemistry: 5 μg/mL. See protocol below.
Optimal working dilutions must be determined by the end user.
Suggested Protocol for Immunohistochemical:
1. Fix cryostat sections with a 1:1 chloroform-acetone mixture for 10 minutes at room temperature.
2. Air Dry.
3. Wash with 2 mL of 1x PBS.
4. Add 200 μL of first antibody (5 μg/mL) to each slide. Incubate for 1 hour at room temperature in a humidity chamber.
5. Wash with 5 mL of 1x PBS.
6. Add appropriately diluted anti-rabbit secondary antibody conjugate and incubate for 1 hour in a humidity chamber
7. Wash with 5 mL of 1x PBS.
8. Add 200 μL of development solution (containing DAB or other substrate depending on the secondary antibody) and incubate at room temperature for the necessary time to develop the dark color. Follow the reaction with a microscope, to establish the correct color intensity.
9. Block the reaction by washing with 2 mL of 1x PBS.
10. Counterstain with hematoxylin as desired.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
N Basora et al.
The Journal of biological chemistry, 274(42), 29819-29825 (1999-10-09)
Integrins are important mediators of cell-laminin interactions. In the small intestinal epithelium, which consists of spatially separated proliferative and differentiated cell populations located, respectively, in the crypt and on the villus, laminins and laminin-binding integrins are differentially expressed along the
Differential expression of integrin mRNAs and proteins during normal rat mammary gland development and in carcinogenesis.
R Y Huang, M M Ip, R Y Huang, M M Ip
Cell and Tissue Research null
Human bronchial epithelial cells secrete laminin 5, express hemidesmosomal proteins, and assemble hemidesmosomes.
P H Michelson, M Tigue, J C Jones
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
Upregulation of a functional form of the beta4 integrin subunit in colorectal cancers correlates with c-Myc expression.
Ni, H; Dydensborg, AB; Herring, FE; Basora, N; Gagne, D; Vachon, PH; Beaulieu, JF
Oncogene null
Modes of regulation of laminin-5 production by rat astrocytes.
S Wagner, H Gardner
Neuroscience Letters null
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