AB1632

Anti-Nitric Oxide Synthase I Antibody

Chemicon^®, from rabbit

• 別名: NOS I, bNOS, nNOS
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: ELISA; IHC; WB
• 化学種の反応性: bovine, mouse, rat
• テクニック: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• citations: 6

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: bovine, mouse, rat
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NM_000620.1
• UniProtアクセッション番号: P29475
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Nos1(18125)

詳細

特異性

Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.

免疫原

Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
酸化ストレス

Anti-Nitric Oxide Synthase I Antibody is an antibody against Nitric Oxide Synthase I for use in ELISA, WB, IH.

Immunohistochemistry: Use at 5 - 10 μg/mL on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4°C with a 1:100 - 1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons.

Western blot: 1 - 10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein.

ELISA: Use at 1.0 μg/mL or lower on plates coated with 50 - 100 ng of peptide antigen.

Optimal working dilutions must be determined by the end user.

物理的形状

Peptide affinity-purified immunoglobulin. Supplied at a 1mg/mL solution in PBS, pH 7.4, containing 0.1% BSA.

保管および安定性

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated thawing and refreezing.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable