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由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
mouse, human, rat
メーカー／製品名
Chemicon®
テクニック
flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
特異性
Recognizes Delta opioid receptor. The control peptide (catalog number AG288) is available for use in antibody blocking studies.
免疫原
Delta opioid receptor N-terminal peptide (LVPSARAELQSSPLV).
Epitope: N-terminus
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経炎症及び疼痛
神経炎症及び疼痛
Anti-Opioid Receptor Antibody, δ, pain, N-terminus detects level of Opioid Receptor & has been published & validated for use in FC, IH(P) & WB.
Immunohistochemistry on CNS tissues fixed in PLP: 1:1,000.
Reactive on paraffin embedded tissue.
Western Blot: 1:1000. Under reducing conditions the antibody will detect major bands at 36 and 72 kDa.
FACS on human T-cells (Sharp, 2001)
Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1560
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (without Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Reactive on paraffin embedded tissue.
Western Blot: 1:1000. Under reducing conditions the antibody will detect major bands at 36 and 72 kDa.
FACS on human T-cells (Sharp, 2001)
Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1560
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (without Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
ターゲットの説明
36 and 72 kDa
関連事項
Replaces: MAB5330
物理的形状
Unpurified
UnPurified whole rabbit antiSerum containing no preservatives.
保管および安定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
アナリシスノート
Control
Spinal cord
Spinal cord
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB1560:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Comparison of immunoblotted delta opioid receptor proteins expressed in the adult rat brain and their regulation by growth hormone.
Persson, Anders I, et al.
Neuroscience Research, 52, 1-9 (2005)
Peripheral ?-opioid receptors attenuate the exercise pressor reflex.
Leal, AK; Yamauchi, K; Kim, J; Ruiz-Velasco, V; Kaufman, MP
American Journal of Physiology. Heart and Circulatory Physiology null
Glial-restricted precursors: patterns of expression of opioid receptors and relationship to human immunodeficiency virus-1 Tat and morphine susceptibility in vitro.
S K Buch, V K Khurdayan, S E Lutz, P E Knapp, N El-Hage, K F Hauser
Neuroscience null
Yuan Xu et al.
Aging, 12(24), 25035-25059 (2020-11-17)
Our previous studies have shown that the δ-opioid receptor (DOR) is an important neuroprotector via the regulation of PTEN-induced kinase 1 (PINK1), a mitochondria-related molecule, under hypoxic and MPP+ insults. Since mitochondrial dysfunctions are observed in both hypoxia and MPP+
Noufissa Kabli et al.
Pain, 127(1-2), 84-93 (2006-09-12)
The analgesic effects of local administration of opioid agonists into peripheral tissues in alleviating pain have been well documented in both clinical and preclinical studies, although few studies have examined their effects in neuropathic pain. In this study, we investigated
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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